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Prolonged oral administration of Gastrodia elata extract improves spatial learning and memory of scopolamine-treated rats
Laboratory Animal Research volume 31, pages69–77(2015)
Gastrodia elata (GE) is traditionally used for treatment of various disorders including neurodegenerative diseases such as Alzheimer’s disease. To investigate the neuroprotective effect of GE, amyloid-β peptide (Aβ)-treated PC12 cells were cultured with GE aqueous extract. In vitro assay demonstrated that 50 µM of pre-aggregated Aβ was lethal to about a half portion of PC12 cells and that Aβ aggregate-induced cell death was significantly decreased with GE treatment at ≤10 mg/mL in a dose-dependent manner. To further examine in vivo cognitive-improving effects, an artificial amnesic animal model, scopolamineinjected Sprague-Dawley rats, were orally administered the extract for 6 weeks followed by behavioral tests (the passive avoidance test and Morris water maze test). The results showed that an acute treatment with scopolamine (1 mg/kg of body weight) effectively induced memory impairment in normal rats and that the learning and memory capability of scopolamine-treated rats improved after prolonged administration of GE extract (50, 250 and 500 mg/kg of body weight for 6 weeks). These findings suggest that a GE regimen may potentially ameliorate learning and memory deficits and/or cognitive impairments caused by neuronal cell death.
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This study was supported by the Core Technology Development and Commercialization Support in Industry-Academia-Research Collaboration (project no. 2013C03) funded by the province of Jeonbuk, Korea.
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Park, Y., Lee, B., Park, S. et al. Prolonged oral administration of Gastrodia elata extract improves spatial learning and memory of scopolamine-treated rats. Lab Anim Res 31, 69–77 (2015). https://doi.org/10.5625/lar.2015.31.2.69
- Gastrodia elata
- scopolamine-induced memory impairment
- amyloid-β peptide
- neuroprotective effect
- cognitive-enhancing effect