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Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging

Laboratory Animal Research volume 31, pages 148–152 (2015)Cite this article

Abstract

In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex® SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 µg/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 µg/mL. High concentrations (≥250 µg/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 µg/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 µM, reaching 80% inhibition at 125 µM. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.

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Acknowledgment

This research was supported by High Value-Added Food Technology Development Program, Ministry of Agriculture, Food and Rural Affairs (MAFRA; grant number 113034-3).

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  1. These two authors equally contributed to this work.

Authors and Affiliations

  1. College of Veterinary Medicine, Chungbuk National University, 1 Chungdaero (Gaesin-dong), Cheongju, Chungbuk, 28644, Korea

    Ehn-Kyoung Choi, Haiyu Guo, Kyungha Shin, Ye-Seul Cha, Youngjin Choi, Da-Woom Seo & Yun-Bae Kim

  2. Central Research Institute, Dr. Chung’s Food Co. Ltd., Cheongju, Korea

    Jae-Kwon Choi & Yoon-Bok Lee

  3. Department of Marine Molecular Biotechnology, Gangneung-Wonju National University, 7 Jukheongil (Jibyeon-dong), Gangneung, Gangwon, 25457, Korea

    Su-Kil Jang & Seong-Soo Joo

Authors
  1. Ehn-Kyoung Choi
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  2. Haiyu Guo
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Correspondence to Seong-Soo Joo or Yun-Bae Kim.

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Choi, EK., Guo, H., Choi, JK. et al. Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging. Lab Anim Res 31, 148–152 (2015). https://doi.org/10.5625/lar.2015.31.3.148

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  • DOI: https://doi.org/10.5625/lar.2015.31.3.148

Keywords

  • Rosa hybrida
  • white rose petal extract
  • matrix metalloproteinase-1
  • elastase
  • tyrosinase

Laboratory Animal Research

ISSN: 2233-7660