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Curcumin attenuates the middle cerebral artery occlusion-induced reduction in γ-enolase expression in an animal model

Laboratory Animal Research volume 31, pages 198–203 (2015)Cite this article

Abstract

Curcumin exerts a protective effect in cerebral ischemia through its anti-oxidant and anti-inflammatory activities. γ-enolase is a glycolytic enzyme expressed in neurons that is known to exerts a neuroprotective effect. We investigated whether curcumin regulates γ-enolase expression in focal cerebral ischemic injury in rats. Middle cerebral artery occlusion (MCAO) was performed to induce focal cerebral ischemia. Adult male rats were injected intraperitoneally with either vehicle or curcumin (50 mg/kg) 1 h after MCAO and cerebral cortex tissues were isolated 24 h after MCAO. We found that MCAO-induced injury resulted in a reduction in γ-enolase expression in vehicle-treated animals using a proteomics approach. However, this reduction was attenuated in animals with MCAO treated with curcumin. Reverse-transcription PCR and Western blot analyses also showed that curcumin treatment prevented the MCAO injury-induced reduction in γ-enolase expression. The results of this study suggest that curcumin exerts its neuroprotective function in focal cerebral ischemia by regulating the expression of γ-enolase.

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  1. These two authors contributed equally to this work.

Authors and Affiliations

  1. Department of Anatomy, College of Veterinary Medicine, Gyeongsang National University, 900 Gajwa-dong, Jinju, Gyeongnam, 660-701, Korea

    Sang-Ah Gim, So-Ra Lee, Fawad-Ali Shah & Phil-Ok Koh

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  1. Sang-Ah Gim
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  2. So-Ra Lee
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  3. Fawad-Ali Shah
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  4. Phil-Ok Koh
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Correspondence to Phil-Ok Koh.

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Gim, SA., Lee, SR., Shah, FA. et al. Curcumin attenuates the middle cerebral artery occlusion-induced reduction in γ-enolase expression in an animal model. Lab Anim Res 31, 198–203 (2015). https://doi.org/10.5625/lar.2015.31.4.198

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  • DOI: https://doi.org/10.5625/lar.2015.31.4.198

Keywords

Laboratory Animal Research

ISSN: 2233-7660