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Role of mitogen-activated protein kinases and nuclear factor-kappa B in 1,3-dichloro-2-propanol-induced hepatic injury

Laboratory Animal Research volume 32, pages 24–33 (2016)Cite this article

Abstract

In this study, the potential hepatotoxicity of 1,3-dichloro-2-propanol and its hepatotoxic mechanisms in rats was investigated. The test chemical was administered orally to male rats at 0, 27.5, 55, and 110 mg/kg body weight. 1,3-Dichloro-2-propanol administration caused acute hepatotoxicity, as evidenced by an increase in serum aminotransferases, total cholesterol, and total bilirubin levels and a decrease in serum glucose concentration in a dose-dependent manner with corresponding histopathological changes in the hepatic tissues. The significant increase in malondialdehyde content and the significant decrease in glutathione content and antioxidant enzyme activities indicated that 1,3-dichloro-2-propanol-induced hepatic damage was mediated through oxidative stress, which caused a dose-dependent increase of hepatocellular apoptotic changes in the terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling assay and immunohistochemical analysis for caspase-3. The phosphorylation of mitogen-activated protein kinases caused by 1,3-dichloro-2-propanol possibly involved in hepatocellular apoptotic changes in rat liver. Furthermore, 1,3-dichloro-2-propanol induced an inflammatory response through activation of nuclear factor-kappa B signaling that coincided with the induction of pro-inflammatory mediators or cytokines in a dose-dependent manner. Taken together, these results demonstrate that hepatotoxicity may be related to oxidative stress-mediated activation of mitogen-activated protein kinases and nuclear factor-kappa B-mediated inflammatory response.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. BK21 Plus Team, College of Veterinary Medicine, Chonnam National University, Gwangju, 500-757, Korea

    In-Chul Lee, Sang-Min Lee, Je-Won Ko, Sung-Hyeuk Park, In-Sik Shin, Changjong Moon, Sung-Ho Kim & Jong-Choon Kim

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  1. In-Chul Lee
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  2. Sang-Min Lee
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  3. Je-Won Ko
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  4. Sung-Hyeuk Park
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Correspondence to Jong-Choon Kim.

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Lee, IC., Lee, SM., Ko, JW. et al. Role of mitogen-activated protein kinases and nuclear factor-kappa B in 1,3-dichloro-2-propanol-induced hepatic injury. Lab Anim Res 32, 24–33 (2016). https://doi.org/10.5625/lar.2016.32.1.24

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  • DOI: https://doi.org/10.5625/lar.2016.32.1.24

Keywords

  • 1,3-dichloro-2-propanol
  • hepatotoxicity
  • MAPKs
  • NF-κB

Laboratory Animal Research

ISSN: 2233-7660