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Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation

Laboratory Animal Research volume 32, pages 34–45 (2016)Cite this article

Abstract

Asparagus cochinchinensis has been used to treat various diseases including fever, cough, kidney disease, breast cancer, inflammatory disease and brain disease, while IL-4 cytokine has been considered as key regulator on the skin homeostasis and the predisposition toward allergic skin inflammation. However, few studies have investigated its effects and IL-4 correlation on skin inflammation to date. To quantitatively evaluate the suppressive effects of ethyl acetate extracts of A. cochinchinensis (EaEAC) on phthalic anhydride (PA)-induced skin inflammation and investigate the role of IL-4 during their action mechanism, alterations in general phenotype biomarkers and luciferase-derived signals were measured in IL-4/Luc/CNS-1 transgenic (Tg) mice with PA-induced skin inflammation after treatment with EaEAC for 2 weeks. Key phenotype markers including lymph node weight, immunoglobulin E (IgE) concentration, epidermis thickness and number of infiltrated mast cells were significantly decreased in the PA+EaEAC treated group compared with the PA+Vehicle treated group. In addition, expression of IL-1ß and TNF-a was also decreased in the PA+EaEAC cotreated group, compared to PA+Vehicle treated group. Furthermore, a significant decrease in the luciferase signal derived from IL-4 promoter was detected in the abdominal region, submandibular lymph node and mesenteric lymph node of the PA+EaEAC treated group, compared to PA+Vehicle treated group. Taken together, these results suggest that EaEAC treatment could successfully improve PA-induced skin inflammation of IL-4/Luc/CNS-1 Tg mice, and that IL-4 cytokine plays a key role in the therapeutic process of EaEAC.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup, Miryang, Gyeongnam, 627-706, Korea

    Ji-Eun Sung, Hyun-Ah Lee, Ji-Eun Kim, Jun Go, Eun-Ji Seo, Woo-Bin Yun, Dong-Seob Kim, Hong-Joo Son & Dae-Youn Hwang

  2. Gangrim Organics, Miryang, 627-706, Korea

    Chung-Yeoul Lee

  3. College of Human Ecology, Pusan National University, Busan, Korea

    Hee-Seob Lee

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  1. Ji-Eun Sung
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Correspondence to Dae-Youn Hwang.

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Sung, JE., Lee, HA., Kim, JE. et al. Therapeutic effect of ethyl acetate extract from Asparagus cochinchinensis on phthalic anhydride-induced skin inflammation. Lab Anim Res 32, 34–45 (2016). https://doi.org/10.5625/lar.2016.32.1.34

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  • DOI: https://doi.org/10.5625/lar.2016.32.1.34

Keywords

Laboratory Animal Research

ISSN: 2233-7660