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Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria
Laboratory Animal Research volume 32, pages 87–90 (2016)
The antibacterial activities of the essential oil of Eucalyptus globulus (EOEG) was determined against 7 fish pathogenic bacteria (Edwardsiella tarda, Streptococcus iniae, S. parauberis, Lactococcus garviae, Vibrio harveyi, V. ichthyoenteri and Photobacterium damselae) obtained from farmed olive flounder. The inhibitory activity was evaluated by three methods: Disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). According to the disc diffusion test, as the concentration of EOEG (5-40 u.g) rises, the inhibitory zone increases in size. Compared with amoxicillin, tetracycline and chloramphenicol, EOEG showed similar antibacterial activity. The MIC of EOEG ranged from 7.8 to 125 mg/mL and MBC values ranged from 62 to 250 mg/mL. These results show that EOEG has antimicrobial activity against all seven bacteria, but there was no marked difference between each genus. From these results, it is suggested that EOEG can be used as an antimicrobial agent against fish bacterial diseases in the fish industry.
Oh MJ, Jung SJ, Kitamura SI, Kim HJ. Kang, SY. Viral diseases of olive flounder in Korean hatcheries. J Ocean Univ China 2006; 5: 45–48.
Goldburg R, Naylor, L. Future seascapes, fishing, and fish farming. Front. Ecol. Environ. 2005; 3: 21–28.
Sung CH, Chun JH, Hyun JY, Hwang IG, Kwak HS, Yoon SH, Lee JS, Chung YH, Song KY, Seo KH. Prevalence and antimicrobial resistance of Vibrio parahaemolyticus isolated from raw fshes. Korean J Vet Public Health 2010; 34(3): 197–202.
Kim KH, Kim AR, Cho EJ, Joo SJ, Park JH, Moon JY, Yum JH, Kim TH, Kwon HJ, Lee HT, Kim YM, Lee EW. Antibacterial activity of Rhus javanica against the Fish Pathogens Vibrio ichthyoenteri and Streptococcus iniae. Kor J Fish Aquat Sci 2014; 47(1): 18–22.
Han SM, Lee KG, Park KK. Antimicrobial activity of honeybee venom against fish pathogenic bacteria. J Fish Pathol 2011; 24(2): 113–120.
Cho JY, Choi I, Hwang EK. Antimicrobacterial activity of extracts from medicinal herbs against Escherichia coli. Korea J Vet Res 2003; 43(4): 625–632.
Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils—a review. Food Chem Toxicol 2008; 46(2): 446–475.
Bruneton J. Pharmacognosy, Phytochemistry, Medicinal Plants. 2nd ed, Lavoisier Publishing, Paris, 1999; pp 405–467.
Egawa H, Tsutsui O, Tatsuyama K, Hatta T. Antifungal substances found in leaves of Eucalyptus species. Experientia 1977; 33(7): 889–890.
Suri RK, Mehra SN. Chemical examination of oils of some eucalyptus. Indian Perfumer 1991; 35(1): 8–12.
Nishimura H, Mizutani J. Economically useful ingredients and the clonal propagation of Eucalyptus citriodora plant. Proc. Hokkaido Tokai University, Science and Engineering 1989; 2: 57–65.
Wyk BEV, Wink M. Medicinal Plants of the World. An Illustrated Scientific Guide to Important Medicinal Plants and Their Uses, 1st ed, Timber Press, Portland, OR, 2004: pp 480.
Cabello FC. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ Microbiol 2006; 8(7): 1137–1144.
Lis-Balchin M, Deans SG, Eaglesham E. Relationship between bioactivity and chemical composition of commercial essential oils. Flavour Frag J 1998; 13(2): 98–104.
Djenane D, Yangela J, Amrouche T, Boubrit S, Boussad N, Roncals P. Chemical composition and antimicrobial effects of essential oils of Eucalyptus globulus, Myrtus communis and Satureja hortensis against Escherichia coli 0157:H7 and Staphylococcus aureus in minced beef. Food Sci Technol Int 2011; 17(6): 505–515.
Mulyaningsih S, Sporer F, Reichling J, Wink M. Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Pharm Biol 2011; 49(9): 893–899.
Klocke JA, Darlington MV, Balandrin MF. 1,8-Cineole (Eucalyptol), a mosquito feeding and ovipositional repellent from volatile oil of Hemizonia fitchii (Asteraceae). J Chem Ecol 1987; 13(12): 2131–2141.
Santos FA, Silva RM, Campos AR, De Arajo RP, Lima Jnior RC, Rao VS. 1,8-cineole (eucalyptol), a monoterpene oxide attenuates the colonic damage in rats on acute TNBS-colitis. Food Chem Toxicol 2004; 42(4): 579–584.
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Park, JW., Wendt, M. & Heo, GJ. Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria. Lab Anim Res 32, 87–90 (2016). https://doi.org/10.5625/lar.2016.32.2.87
- Eucalyptus globulus
- antibacterial activity
- fish pathogenic bacteria