Skip to main content

Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria


The antibacterial activities of the essential oil of Eucalyptus globulus (EOEG) was determined against 7 fish pathogenic bacteria (Edwardsiella tarda, Streptococcus iniae, S. parauberis, Lactococcus garviae, Vibrio harveyi, V. ichthyoenteri and Photobacterium damselae) obtained from farmed olive flounder. The inhibitory activity was evaluated by three methods: Disc diffusion method, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). According to the disc diffusion test, as the concentration of EOEG (5-40 u.g) rises, the inhibitory zone increases in size. Compared with amoxicillin, tetracycline and chloramphenicol, EOEG showed similar antibacterial activity. The MIC of EOEG ranged from 7.8 to 125 mg/mL and MBC values ranged from 62 to 250 mg/mL. These results show that EOEG has antimicrobial activity against all seven bacteria, but there was no marked difference between each genus. From these results, it is suggested that EOEG can be used as an antimicrobial agent against fish bacterial diseases in the fish industry.


  1. Oh MJ, Jung SJ, Kitamura SI, Kim HJ. Kang, SY. Viral diseases of olive flounder in Korean hatcheries. J Ocean Univ China 2006; 5: 45–48.

    Article  Google Scholar 

  2. Goldburg R, Naylor, L. Future seascapes, fishing, and fish farming. Front. Ecol. Environ. 2005; 3: 21–28.

    Article  Google Scholar 

  3. Sung CH, Chun JH, Hyun JY, Hwang IG, Kwak HS, Yoon SH, Lee JS, Chung YH, Song KY, Seo KH. Prevalence and antimicrobial resistance of Vibrio parahaemolyticus isolated from raw fshes. Korean J Vet Public Health 2010; 34(3): 197–202.

    Google Scholar 

  4. Kim KH, Kim AR, Cho EJ, Joo SJ, Park JH, Moon JY, Yum JH, Kim TH, Kwon HJ, Lee HT, Kim YM, Lee EW. Antibacterial activity of Rhus javanica against the Fish Pathogens Vibrio ichthyoenteri and Streptococcus iniae. Kor J Fish Aquat Sci 2014; 47(1): 18–22.

    CAS  Google Scholar 

  5. Han SM, Lee KG, Park KK. Antimicrobial activity of honeybee venom against fish pathogenic bacteria. J Fish Pathol 2011; 24(2): 113–120.

    Article  Google Scholar 

  6. Cho JY, Choi I, Hwang EK. Antimicrobacterial activity of extracts from medicinal herbs against Escherichia coli. Korea J Vet Res 2003; 43(4): 625–632.

    Google Scholar 

  7. Bakkali F, Averbeck S, Averbeck D, Idaomar M. Biological effects of essential oils—a review. Food Chem Toxicol 2008; 46(2): 446–475.

    CAS  Article  Google Scholar 

  8. Bruneton J. Pharmacognosy, Phytochemistry, Medicinal Plants. 2nd ed, Lavoisier Publishing, Paris, 1999; pp 405–467.

    Google Scholar 

  9. Egawa H, Tsutsui O, Tatsuyama K, Hatta T. Antifungal substances found in leaves of Eucalyptus species. Experientia 1977; 33(7): 889–890.

    CAS  Article  Google Scholar 

  10. Suri RK, Mehra SN. Chemical examination of oils of some eucalyptus. Indian Perfumer 1991; 35(1): 8–12.

    CAS  Google Scholar 

  11. Nishimura H, Mizutani J. Economically useful ingredients and the clonal propagation of Eucalyptus citriodora plant. Proc. Hokkaido Tokai University, Science and Engineering 1989; 2: 57–65.

    Google Scholar 

  12. Wyk BEV, Wink M. Medicinal Plants of the World. An Illustrated Scientific Guide to Important Medicinal Plants and Their Uses, 1st ed, Timber Press, Portland, OR, 2004: pp 480.

    Google Scholar 

  13. Cabello FC. Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environ Microbiol 2006; 8(7): 1137–1144.

    CAS  Article  Google Scholar 

  14. Lis-Balchin M, Deans SG, Eaglesham E. Relationship between bioactivity and chemical composition of commercial essential oils. Flavour Frag J 1998; 13(2): 98–104.

    CAS  Article  Google Scholar 

  15. Djenane D, Yangela J, Amrouche T, Boubrit S, Boussad N, Roncals P. Chemical composition and antimicrobial effects of essential oils of Eucalyptus globulus, Myrtus communis and Satureja hortensis against Escherichia coli 0157:H7 and Staphylococcus aureus in minced beef. Food Sci Technol Int 2011; 17(6): 505–515.

    CAS  Article  Google Scholar 

  16. Mulyaningsih S, Sporer F, Reichling J, Wink M. Antibacterial activity of essential oils from Eucalyptus and of selected components against multidrug-resistant bacterial pathogens. Pharm Biol 2011; 49(9): 893–899.

    CAS  Article  Google Scholar 

  17. Klocke JA, Darlington MV, Balandrin MF. 1,8-Cineole (Eucalyptol), a mosquito feeding and ovipositional repellent from volatile oil of Hemizonia fitchii (Asteraceae). J Chem Ecol 1987; 13(12): 2131–2141.

    CAS  Article  Google Scholar 

  18. Santos FA, Silva RM, Campos AR, De Arajo RP, Lima Jnior RC, Rao VS. 1,8-cineole (eucalyptol), a monoterpene oxide attenuates the colonic damage in rats on acute TNBS-colitis. Food Chem Toxicol 2004; 42(4): 579–584.

    CAS  Article  Google Scholar 

Download references

Author information

Authors and Affiliations


Corresponding author

Correspondence to Gang-Joon Heo.

Rights and permissions

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License ( which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Park, JW., Wendt, M. & Heo, GJ. Antimicrobial activity of essential oil of Eucalyptus globulus against fish pathogenic bacteria. Lab Anim Res 32, 87–90 (2016).

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI:


  • Eucalyptus globulus
  • antibacterial activity
  • fish pathogenic bacteria