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Prevalence of Salmonella spp. in pet turtles and their environment
Laboratory Animal Research volume 32, pages166–170(2016)
Pet turtles are known as a source of Salmonella infection to humans when handled in captivity. Thirty four turtles purchased from pet shops and online markets in Korea were examined to determine whether the turtles and their environment were contaminated with Salmonella spp. Salmonella spp. were isolated from fecal samples of 17 turtles. These isolates were identified as S. enterica through 16S rRNA gene sequencing. The isolation rate of Salmonella spp. from the soil and water samples increased over time. We concluded that a high percentage of turtles being sold in pet shops were infected with Salmonella spp., and their environments tend to become contaminated over time unless they are maintained properly. These results indicate that pet turtles could be a potential risk of salmonellosis in Korea.
Uzzau S, Brown DJ, Wallis T, Rubino S, Leori G, Bernard S, Casadesus J, Platt DJ, Olsen JE. Host adapted serotypes of Salmonella enterica. Epidemiol Infect 2000; 125(2): 229–255.
Minette HP. Epidemiologic aspects of salmonellosis in reptiles, amphibians, mollusks and crustaceans—a review. Int J Zoonoses 1984; 11(1): 95–104.
Pfleger S, Benyr G, Sommer R, Hassl A. Pattern of Salmonella excretion in amphibians and reptiles in a vivarium. Int J Hyg Environ Health 2003; 206(1): 53–59.
Sanyal D, Douglas T, Roberts R. Salmonella infection acquired from reptilian pets. Arch Dis Child 1997; 77(4): 345–346.
Ward L. Salmonella perils of pet reptiles. Commun Dis Public Health 2000; 3(1): 2–3.
Cambre RC, Green DE, Smith EE, Montali RJ, Bush M. Salmonellosis and arizonosis in the reptile collection at the National Zoological Park. J Am Vet Med Assoc 1980; 177(9): 800–803.
Corrente M, Madio A, Friedrich KG, Greco G, Desario C, Tagliabue S, D’Incau M, Campolo M, Buonavoglia C. Isolation of Salmonella strains from reptile faeces and comparison of different culture media. J Appl Microbiol 2004; 96(4): 709–715.
Pasmans F, De Herdt P, Dewulf J, Haesebrouck F. Pathogenesis of infections with Salmonella enterica subsp. enterica serovar Muenchen in the turtle Trachemys scripta scripta. Vet Microbiol 2002; 87(4): 315–325.
Benenson AS. Control of communicable diseases manual, 16th ed. American Public Health Association, Washington, D.C, 1995; pp 1–577.
Yun YS, Lee DY, Chung GT. Prevalence and characteristics of Salmonella spp. isolated in Korea. Official public of KCDC 2015; 9(10): 174–180.
Woodward DL, Khakhria R, Johnson WM. Human salmonellosis associated with exotic pets. J Clin Microbiol 1997; 35(11): 2786–2790.
Kaufmann AF, Feeley JC, DeWitt WE. Salmonella excretion by turtles. Public Health Rep 1967; 82(9): 840–842.
Nowakiewicz A, Ziolkowska G, Zieba P, Stepniewska K, Tokarzewski S. Russian tortoises (Agrionemys horsfieldi) as a potential reservoir for Salmonella spp. Res Ve t Sci 2012; 92(2): 187–190.
Wells EV, Boulton M, Hall W, Bidol SA. Reptile-associated salmonellosis in preschool-aged children in Michigan, January 2001-June 2003. Clin Infect Dis 2004; 39(5): 687–691.
Wybo I, Potters D, Plaskie K, Covens L, Collard JM, Lauwers S. Salmonella enterica subspecies houtenae serotype 44:z4, z23:—as a rare cause of meningitis. Acta Clin Belg 2004; 59(4): 232–234.
Kim JD, Choi SY, Kim DS, Kim KH. Two children with nontyphoidal salmonellosis assumed by pets. Korean J Pediatr Infect Dis 2013; 20(1): 41–45.
Readel AM, Phillips CA, Goldberg TL. Prevalence of Salmonella in intestinal mucosal samples from free-ranging red-eared sliders (Trachemys scripta) in illinois. Herpetol Conserv Biol 2008; 5(2): 207–213.
Chiodini RJ, Sundberg JP. Salmonellosis in reptiles: a review. Am J Epidemiol 1981; 113(5): 494–499.
Hardy T. The tortoise and the scare: Does the potential threat of Salmonella contamination warrant the end of the pet turtle? Bioscience 1988; 38: 76–79.
Lee DY, Chae SJ. Salmonella infection from small turtles in the USA and Republic of Korea. Official public of KCDC 2013; 6(51): 1037–1043.
Bauwens L, Vercammen F, Bertrand S, Collard JM, De Ceuster S. Isolation of Salmonella from environmental samples collected in the reptile department of Antwerp Zoo using different selective methods. J Appl Microbiol 2006; 101(2): 284–289.
Dalton C, Hoffman R, Pape J. Iguana-associated salmonellosis in children. Pediatr Infect Dis J 1995; 14(4): 319–320.
Siebeling RJ, Neal PM, Granberry WD. Evaluation of methods for the isolation of Salmonella and Arizona organisms from pet turtles treated with antimicrobial agents. Appl Microbiol 1975; 29(2): 240–245.
Jang YH, Lee SJ, Lim JG, Lee HS, Kim TJ, Park JH, Chung BH, Choe NH. The rate of Salmonella spp. infection in zoo animals at Seoul Grand Park, Korea. J Ved Sci 2008; 9(2): 177–181.
Meyer Sauteur PM, Relly C, Hug M, Wittenbrink MM, Berger C. Risk factors for invasive reptile-associated salmonellosis in children. Vector Borne Zoonotic Dis 2013; 13(6): 419–421.
Friedman CR, Torigian C, Shillam PJ, Hoffman RE, Heltzel D, Beebe JL, Malcolm G, DeWitt WE, Hutwagner L, Griffin PM. An outbreak of salmonellosis among children attending a reptile exhibit at a zoo. J Pediatr 1998; 132(5): 802–807.
Mitchell MA, Shane SM. Preliminary findings of Salmonella spp. in captive green iguanas (Iguana iguana) and their environment. Prev Vet Med 2000; 45(3-4): 297–304
Hidalgo-Vila J, Diaz-Paniagua C, Perez-Santigosa N, de Frutos-Escobar C, Herrero-Herrero A. Salmonella in free-living exotic and native turtles and in pet exotic turtles from SW Spain. Res Vet Sci 2008; 85(3): 449–452.
Nakadai A, Kuroki T, Kato Y, Suzuki R, Yamai S, Yaginuma C, Shiotani R, Yamanouchi A, Hayashidani H. Prevalence of Salmonella spp. in pet reptiles in Japan. J Vet Med Sci 2005; 67(1): 97–101.
Burnham BR, Atchley DH, DeFusco RP, Ferris KE, Zicarelli JC, Lee JH, Angulo FJ. Prevalence of fecal shedding of Salmonella organisms among captive green iguanas and potential public health implications. J Am Vet Med Assoc 1998; 213(1): 48–50.
DuPonte MW, Nakamura RM, Chang EM. Activation of latent Salmonella and Arizona organisms by dehydration of red-eared turtles, Pseudemys scripta- elegans. Am J Vet Res 1978; 39(3): 529–530.
Grimont PAD, Weill FX. Antigenic formulae of the Salmonella serovars, 9th ed, Institut Pasteur, Paris, 2007; pp 1–13.
This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1D1A1A01060638).
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Back, D., Shin, G., Wendt, M. et al. Prevalence of Salmonella spp. in pet turtles and their environment. Lab Anim Res 32, 166–170 (2016). https://doi.org/10.5625/lar.2016.32.3.166
- pet turtles
- Salmonella spp.