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Diallyl disulfide attenuates acetaminophen-induced renal injury in rats

Laboratory Animal Research volume 32, pages 200–207 (2016)Cite this article

Abstract

This study investigated the protective effects of diallyl disulfide (DADS) against acetaminophen (AAP)-induced acute renal injury in male rats. We also investigated the effects of DADS on kidney injury molecule-1 (KIM-1) and neutrophil gelatinase-associated lipocalin (NCAL), which are novel biomarkers of nephrotoxicity in renal tissues, in response to AAP treatment. The following four experimental groups were evaluated: (1) vehicle control, (2) AAP (1,000 mg/kg), (3) AAP&DADS, and (4) DADS (50 mg/kg/day). AAP treatment caused acute kidney injury evidenced by increased serum blood urea nitrogen (BUN) levels and histopathological alterations. Additionally, Western blot and immunohistochemistry analysis showed increased expression of KIM-1 and NCAL proteins in renal tissues of AAP-treated rats. In contrast, DADS pretreatment significantly attenuated the AAP-induced nephrotoxic effects, including serum BUN level and expression of KIM-1 and NCAL proteins. Histopathological studies confirmed the renoprotective effect of DADS. The results suggest that DADS prevents AAP-induced acute nephrotoxicity, and that KIM-1 and NCAL may be useful biomarkers for the detection and monitoring of acute kidney injury associated with AAP exposure.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Ministry of Food and Drug Safety, Cheongju, Korea

    Jin-Young Shin

  2. College of Veterinary Medicine, Chonnam National University, Gwangju, 61186, Korea

    Jin-Young Shin, Ji-Hee Han, Je-Won Ko, Sung-Hyeuk Park, Na-Rae Shin, Tae-Yang Jung, Hyun-A Kim, In-Sik Shin & Jong-Choon Kim

  3. Jeonbuk Department of Inhalation Research, Korea Institute of Toxicology, Jeongeup, Korea

    Sung-Hwan Kim

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Correspondence to Jong-Choon Kim.

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Shin, JY., Han, JH., Ko, JW. et al. Diallyl disulfide attenuates acetaminophen-induced renal injury in rats. Lab Anim Res 32, 200–207 (2016). https://doi.org/10.5625/lar.2016.32.4.200

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  • DOI: https://doi.org/10.5625/lar.2016.32.4.200

Keywords

Laboratory Animal Research

ISSN: 2233-7660