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Establishment of a surgically induced cryptorchidism canine recipient model for spermatogonial stem cell transplantation

Laboratory Animal Research volume 32, pages 257–266 (2016)Cite this article

Abstract

Transplantation of spermatogonial stem cells (SSCs) in experimental animal models has been used to study germ line stem cell biology and to produce transgenic animals. The species-specific recipient model preparation is important for the characterization of SSCs and the production of offspring. Here, we investigated the effects of surgically induced cryptorchidism in dog as a new recipient model for spermatogonial stem cell transplantation. Artificially unilateral or bilateral cryptorchidism was induced in ten mature male dogs by surgically returning the testis and epididymis to the abdominal cavity. The testes and epididymides were collected every week after the induction of artificial cryptorchidism (surgery) for one month. To determine the effect of surgical cryptorchidism, the seminiferous tubule diameter was measured and immunohistochemistry using PGP9.5 and GATA4 antibodies was analyzed. The diameters of the seminiferous tubules of abdominal testes were significantly reduced compared to those of the scrotal testes. Immunohistochemistry results showed that PGP9.5 positive undifferentiated spermatogonia were significantly reduced after surgical cryptorchidism induction, but there were no significant changes in GATA-4 positive sertoli cells. To evaluate the testis function recovery rate, orchiopexy was performed on two dogs after 30 days of bilateral cryptorchidism. In the orchiopexy group, SCP3 positive spermatocytes were detected, and spermatogenesis was recovered 8 weeks after orchiopexy. In this study, we provided optimum experimental conditions and time for surgical preparation of a recipient canine model for SSC transplantation. Additionally, our data will contribute to recipient preparation by using surgically induced cryptorchidism in non-rodent species.

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Authors and Affiliations

  1. Department of Food Bioscience, RIBHS, College of Biomedical & Health Science, Konkuk University, Chungju, Korea

    Won-Young Lee & Ran Lee

  2. Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Korea

    Hyuk Song

  3. Animal Biotechnology Division, National Institute of Animal Science, RDA, Jeonju, Korea

    Tai-Young Hur & Seunghoon Lee

  4. Metabolism and Nutrition Research Group, Korea Food Research Institute, Seongnam, Korea

    Jiyun Ahn

  5. Research Group of Nutraceuticals for Metabolic Syndrome, Korea Food Research Institute, Seongnam, Korea

    Hyunjhung Jhun

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  1. Won-Young Lee
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Correspondence to Hyunjhung Jhun.

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Lee, WY., Lee, R., Song, H. et al. Establishment of a surgically induced cryptorchidism canine recipient model for spermatogonial stem cell transplantation. Lab Anim Res 32, 257–266 (2016). https://doi.org/10.5625/lar.2016.32.4.257

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  • DOI: https://doi.org/10.5625/lar.2016.32.4.257

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Laboratory Animal Research

ISSN: 2233-7660