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Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum)

Abstract

The purpose of this study was to investigate the immunomodulatory activity of ice plant (Mesembryanthemum crystallinum) extract (IPE) in vitro and in vivo. Raji (a human B cell line) and Jurkat (a human T cell line) cells were treated with various doses of IPE and cell proliferation was measured by WST assay. Results showed that IPE promoted the proliferation of both Raji and Jurkat cells in a dose-dependent manner. IPE also enhanced IL-6 and TNF-α production in macrophages in the presence of lipopolysaccharide (LPS), although IPE alone did not induce cytokine production. Moreover, IPE treatment upregulated iNOS gene expression in macrophages in a time- and dose-dependent manner and led to the production of nitric oxide in macrophages in the presence of IFNγ. In vivo studies revealed that oral administration of IPE for 2 weeks increased the differentiation of CD4+, CD8+, and CD19+ cells in splenocytes. These findings suggested that IPE has immunomodulatory effects and could be developed as an immunomodulatory supplement.

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Acknowledgment

This study was supported by the Small and Medium Business Administration (SMBA, Grant No. S2357445).

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Correspondence to Jong-Hwan Park.

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This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Choi, JH., Jo, SG., Jung, SK. et al. Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum). Lab Anim Res 33, 32–39 (2017). https://doi.org/10.5625/lar.2017.33.1.32

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  • DOI: https://doi.org/10.5625/lar.2017.33.1.32

Keywords

  • Ice plant extract (IPE)
  • lymphocyte
  • macrophage
  • cytokines
  • cell proliferation