Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum)
Laboratory Animal Research volume 33, pages 32–39 (2017)
Abstract
The purpose of this study was to investigate the immunomodulatory activity of ice plant (Mesembryanthemum crystallinum) extract (IPE) in vitro and in vivo. Raji (a human B cell line) and Jurkat (a human T cell line) cells were treated with various doses of IPE and cell proliferation was measured by WST assay. Results showed that IPE promoted the proliferation of both Raji and Jurkat cells in a dose-dependent manner. IPE also enhanced IL-6 and TNF-α production in macrophages in the presence of lipopolysaccharide (LPS), although IPE alone did not induce cytokine production. Moreover, IPE treatment upregulated iNOS gene expression in macrophages in a time- and dose-dependent manner and led to the production of nitric oxide in macrophages in the presence of IFNγ. In vivo studies revealed that oral administration of IPE for 2 weeks increased the differentiation of CD4+, CD8+, and CD19+ cells in splenocytes. These findings suggested that IPE has immunomodulatory effects and could be developed as an immunomodulatory supplement.
References
Yan ZQ, Hansson GK. Innate immunity, macrophage activation, and atherosclerosis. Immunol Rev 2007; 219: 187–203.
Wang, M.-L., et al, Immunomodulatory activities of Gelidium amansii gel extracts on murine RAW 264.7 macrophages. Journal of Food and Drug Analysis 2013; 21(4): 397–403.
Zhang X, Morrison DC. Lipopolysaccharide-induced selective priming effects on tumor necrosis factor alpha and nitric oxide production in mouse peritoneal macrophages. J Exp Med 1993; 177(2): 511–516.
Naka T, Nishimoto N, Kishimoto T. The paradigm of IL-6: from basic science to medicine. Arthritis Res 2002; 4 Suppl 3: S233- 242.
Bogdan C. Nitric oxide and the immune response. Nat Immunol 2001; 2(10): 907–916.
Prasad S, Sung B, Aggarwal BB. Age-associated chronic diseases require age-old medicine: role of chronic inflammation. Prev Med 2012; 54 Suppl: S29–37.
Miyazaki Y, Kusano S, Doi H, Aki O. Effects on immune response of antidiabetic ingredients from white-skinned sweet potato (Ipomoea batatas L.). Nutrition 2005; 21(3): 358–362.
Lin BF, Chiang BL, Lin JY. Amaranthus spinosus water extract directly stimulates proliferation of B lymphocytes in vitro. Int Immunopharmacol 2005; 5(4): 711–722.
Chen X, Nie W, Yu G, Li Y, Hu Y, Lu J, Jin L. Antitumor and immunomodulatory activity of polysaccharides from Sargassum fusiforme. Food Chem Toxicol 2012; 50(3-4): 695–700.
Thakur M, Connellan P, Deseo MA, Morris C, Praznik W, Loeppert R, Dixit VK. Characterization and in vitro immuno modulatory screening of fructo-oligosaccharides of Asparagus racemosus Willd. Int J Biol Macromol 2012; 50(1): 77–81.
Tsukagoshi H, Suzuki T, Nishikawa K, Agarie S, Ishiguro S, Higashiyama T RNA-seq analysis of the response of the halophyte, Mesembryanthemum crystallinum (ice plant) to high salinity. PLoS One 2015; 10(2): e0118339.
AMARASINGHE V, L. WATSON. Comparative ultrastructure of microhairs in grasses. Botanical Journal of the Linnean Society 1988; 98(4): 303–319.
Vogt T, Ibdah M, Schmidt J, Wray V, Nimtz M, Strack D. Light- induced betacyanin and flavonol accumulation in bladder cells of Mesembryanthemum crystallinum. Phytochemistry 1999; 52(4): 583–592.
Deters AM, Meyer U, Stintzing FC. Time-dependent bioactivity of preparations from cactus pear (Opuntia ficus indica) and ice plant (Mesembryanthemum crystallinum) on human skin fibroblasts and keratinocytes. J Ethnopharmacol 2012; 142(2): 438–444.
Drira R, Matsumoto T, Agawa M, Sakamoto K. Ice Plant (Mesembryanthemum crystallinum) Extract Promotes Lipolysis in Mouse 3T3-L1 Adipocytes Through Extracellular Signal- Regulated Kinase Activation. J Med Food 2016; 19(3): 274–280.
Sivakumar S, Subramanian SP. Pancreatic tissue protective nature of D-Pinitol studied in streptozotocin-mediated oxidative stress in experimental diabetic rats. Eur J Pharmacol 2009; 622(1-3): 65–70.
Lee BH, Lee CC, Wu SC. ce plant (Mesembryanthemum crystallinum) improves hyperglycaemia and memory impairments in a Wistar rat model of streptozotocin-induced diabetes. J Sci Food Agric 2014; 94(11): 2266–2273.
Lin TH, Tan TW, Tsai TH, Chen CC, Hsieh TF, Lee SS, Liu HH, Chen WC, Tang CH. D-pinitol inhibits prostate cancer metastasis through inhibition of αV(53 integrin by modulating FAK, c-Src and NF-KB pathways. Int J Mol Sci 2013; 14(5): 9790–9802.
Singh RK, Pandey BL, Tripathi M, Pandey VB. Anti inflammatory effect of (+)-pinitol. Fitoterapia 2001; 72(2): 168–170.
Celada A, Gray PW, Rinderknecht E, Schreiber RD. Evidence for a gamma-interferon receptor that regulates macrophage tumoricidal activity. J Exp Med 1984; 160(1): 55–74.
Kim H, Lee HS, Chang KT, Ko TH, Baek KJ, Kwon NS. Chloromethyl ketones block induction of nitric oxide synthase in murine macrophages by preventing activation of nuclear factor- kappa B. J Immunol 1995; 154(9): 4741–4748.
Gill HS, Rutherford KJ, Prasad J, Gopal PK. Enhancement of natural and acquired immunity by Lactobacillus rhamnosus (HN001), Lactobacillus acidophilus (HN017) and Bifidobacterium lactis (HN019). Br J Nutr 2000; 83(2): 167–176.
Ghonime M, Emara M, Shawky R, Soliman H, El-Domany R, Abdelaziz A. Immunomodulation of RAW 264.7 murine macrophage functions and antioxidant activities of 11 plant extracts. Immunol Invest 2015; 44(3): 237–252.
Glauser MP. The inflammatory cytokines. New developments in the pathophysiology and treatment of septic shock. Drugs 1996; 52 Suppl 2: 9–17.
Hirohashi N, Morrison DC. Low-dose lipopolysaccharide (LPS) pretreatment of mouse macrophages modulates LPS-dependent interleukin-6 production in vitro. Infect Immun 1996; 64(3): 1011- 1015.
Winthrop KL. Risk and prevention of tuberculosis and other serious opportunistic infections associated with the inhibition of tumor necrosis factor. Nat Clin Pract Rheumatol 2006; 2(11): 602–610.
Boyman O, Hefti HP, Conrad C, Nickoloff BJ, Suter M, Nestle FO. Spontaneous development of psoriasis in anew animal model shows an essential role for resident T cells and tumor necrosis factor-alpha. J Exp Med 2004; 199(5): 731–736.
Kehrl JH, Miller A, Fauci AS. Effect of tumor necrosis factor alpha on mitogen-activated human B cells. J Exp Med 1987; 166(3): 786–791.
Bettelli E, Carrier Y, Gao W, Korn T, Strom TB, Oukka M, Weiner HL, Kuchroo VK. Reciprocal developmental pathways for the generation of pathogenic effector TH17 and regulatory T cells. Nature 2006; 441(7090): 235–238.
Kishimoto T, Akira S, Narazaki M, Taga T. Interleukin-6 family of cytokines and gp130.Blood 1995; 86(4): 1243–1254.
MacMicking J, Xie QW, Nathan C. Nitric oxide and macrophage function. Annu Rev Immunol 1997; 15: 323–350.
Suhkneung, P., et al., Immunomodulatory Effects of Cimicifugae Rhizoma Extracts in Macrophages. Preventive Nutrition and Food Science 2006; 11(4): 268–272.
Swain SL, McKinstry KK, Strutt TM. Expanding roles for CD4T cells in immunity to viruses. Nat Rev Immunol 2012; 12(2): 136–148.
Fujimoto M, Poe JC, Inaoki M, Tedder TF. CD 19 regulates B lymphocyte responses to transmembrane signals. Semin Immunol 1998; 10(4): 267–277.
Han SS, Cho CK, Lee YW, Yoo HS. Antimetastatic and immunomodulating effect of water extracts from various mushrooms. J Acupunct Meridian Stud 2009; 2(3): 218–227.
Acknowledgment
This study was supported by the Small and Medium Business Administration (SMBA, Grant No. S2357445).
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Choi, JH., Jo, SG., Jung, SK. et al. Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum). Lab Anim Res 33, 32–39 (2017). https://doi.org/10.5625/lar.2017.33.1.32
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DOI: https://doi.org/10.5625/lar.2017.33.1.32