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Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum)
Laboratory Animal Research volume 33, pages 32–39 (2017)
The purpose of this study was to investigate the immunomodulatory activity of ice plant (Mesembryanthemum crystallinum) extract (IPE) in vitro and in vivo. Raji (a human B cell line) and Jurkat (a human T cell line) cells were treated with various doses of IPE and cell proliferation was measured by WST assay. Results showed that IPE promoted the proliferation of both Raji and Jurkat cells in a dose-dependent manner. IPE also enhanced IL-6 and TNF-α production in macrophages in the presence of lipopolysaccharide (LPS), although IPE alone did not induce cytokine production. Moreover, IPE treatment upregulated iNOS gene expression in macrophages in a time- and dose-dependent manner and led to the production of nitric oxide in macrophages in the presence of IFNγ. In vivo studies revealed that oral administration of IPE for 2 weeks increased the differentiation of CD4+, CD8+, and CD19+ cells in splenocytes. These findings suggested that IPE has immunomodulatory effects and could be developed as an immunomodulatory supplement.
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This study was supported by the Small and Medium Business Administration (SMBA, Grant No. S2357445).
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Choi, JH., Jo, SG., Jung, SK. et al. Immunomodulatory effects of ethanol extract of germinated ice plant (Mesembryanthemum crystallinum). Lab Anim Res 33, 32–39 (2017). https://doi.org/10.5625/lar.2017.33.1.32
- Ice plant extract (IPE)
- cell proliferation