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Comparison of commonly used ICR stocks and the characterization of Korl:ICR


Mouse is a commonly used animal in life science studies and is classified as outbred if genetically diverse and inbred if genetically homogeneous. Outbred mouse stocks, are used in toxicology, oncology, infection and pharmacology research. The National Institute of Food and Drug Safety Evaluation (NIFDS; former the Korea National Institute of Health) have bred ICR mice for more than 50 years. We investigated to provide users with information and promote accountability to the Korl:ICR. To secure the indigenous data, biological characteristics of Korl:ICR were identified by comparing with other ICR stocks. This domestic ICR stock was denominated as ‘Korl:ICR’. Phylogenetic analysis using SNPs indicated that the population stratification of the Korl:ICR was allocated different area with other ICR. In addition, we measured litter size, body weight, body length, various organ weight, hematology and clinical blood chemistry of the Korl:ICR compared to other ICR. Otherwise, there are no significant differences among the biological phenotypes of Korl:ICR and other ICR. These results suggest that as a genetically indigenous source colony, the Korl:ICR is seperated (or independent) stock with other ICR. Also, we confirmed that there is no difference among the Korl:ICR and other ICR on biological phenotypes. Therefore, the Korl:ICR source colony might be a new stock in distinction from other ICR, it is a good milestone in securing ownership of the national laboratory animal resource. The NIFDS expects that the Korl:ICR mice will be useful animal resource for our domestic researchers.


  1. Cui S, Chesson C, Hope R. Genetic variation within and between strains of outbred Swiss mice. Lab Anim 1993; 27(2): 116–123.

    Article  CAS  PubMed  Google Scholar 

  2. Rice MC, O’Brien SJ. Genetic variance of laboratory outbred Swiss mice. Nature 1980; 283(5743): 157–161.

    Article  CAS  PubMed  Google Scholar 

  3. Chia R, Achilli F, Festing MF, Fisher EM. The origins and uses of mouse outbred stocks. Nat Genet 2005; 37(11): 1181–1186.

    Article  CAS  PubMed  Google Scholar 

  4. Yamada J, Nikaido H, Matsumoto S. Genetic variability within and between outbred Wistar strains of rats. Jikken Dobutsu 1979; 28(2): 259–265.

    CAS  PubMed  Google Scholar 

  5. Phelan JP. Genetic variability and rodent models of human aging. Exp Gerontol 1992; 27(2): 147–159.

    Article  CAS  PubMed  Google Scholar 

  6. DeFries JC, Wilson JR, Erwin VG, Petersen DR. LS X SS recombinant inbred strains of mice: initial characterization. Alcohol Clin Exp Res 1989; 13(2): 196–200.

    Article  CAS  PubMed  Google Scholar 

  7. Feingold N, Feingold J, Mouton D, Bouthillier Y, Stiffel C, Biozzi G. Polygenic regulation of antibody synthesis to sheep erythrocytes in the mouse: a genetic analysis. Eur J Immunol 1976; 6(1): 43–51.

    Article  CAS  PubMed  Google Scholar 

  8. Schlager G. Genetic Hypertension in the Mouse, Elsevier, Amsterdam, 1994: pp 158–172.

    Google Scholar 

  9. Boutwell RK. Some Biological Aspects of Skin Carcinogenisis. Prog Exp Tumor Res 1964; 19(4): 207–250.

    Google Scholar 

  10. Mathews CE, Bagley R, Leiter EH. ALS/Lt: a new type 2 diabetes mouse model associated with low free radical scavenging potential. Diabetes 2004; 53(1 suppl): 125–129.

    Article  Google Scholar 

  11. Garland T Jr, Morgan MT, Swallow JG, Rhodes JS, Girard I, Belter JG, Carter PA. Evolution of a small-muscle polymorphism in lines of house mice selected for high activity levels. Evolution 2002; 56(6): 1267–1275.

    Article  PubMed  Google Scholar 

  12. Kirkpatrick BW, Mengelt A, Schulman N, Martin IC. Identification of quantitative trait loci for prolificacy and growth in mice. Mamm Genome 1998; 9(2): 97–102.

    Article  CAS  PubMed  Google Scholar 

  13. Horvat S, Bünger L, Falconer VM, Mackay P, Law A, Bulfield G, Keightley PD. Mapping of obesity QTLs in a cross between mouse lines divergently selected on fat content. Mamm Genome 2000; 11(1): 2–7.

    Article  CAS  PubMed  Google Scholar 

  14. Crabbe JC, Belknap JK, Buck KJ. Genetic animal models of alcohol and drug abuse. Science 1994; 264(5166): 1715–1723.

    Article  CAS  PubMed  Google Scholar 

  15. Grahame NJ, Li TK, Lumeng L. Selective breeding for high and low alcohol preference in mice. Behav Genet 1999; 29(1): 47–57.

    Article  CAS  PubMed  Google Scholar 

  16. Lynch CJ. The so-called Swiss mouse. Lab Anim Care 1969; 19(2): 214–220.

    CAS  PubMed  Google Scholar 

  17. Keenan KP, Smith PF, Hertzog P, Soper K, Ballam GC, Clark RL. The effects of overfeeding and dietary restriction on Sprague-Dawley rat survival and early pathology biomarkers of aging. Toxicol Pathol 1994; 22(3): 300–315.

    Article  CAS  PubMed  Google Scholar 

  18. Keenan KP, Ballam GC, Dixit R, Soper KA, Laroque P, Mattson BA, Adams SP, Coleman JB. The effects of diet, overfeeding and moderate dietary restriction on Sprague-Dawley rat survival, disease and toxicology. J Nutr 1997; 127(5 suppl): 851S–856S.

    Article  CAS  PubMed  Google Scholar 

  19. Kacew S, Ruben Z, McConnell RF. Strain as a determinant factor in the differential responsiveness of rats to chemicals. Toxicol Pathol 1995; 23(6): 701–714.

    Article  CAS  PubMed  Google Scholar 

  20. Kacew S, Ruben Z. Importance of confounding factors including strain, sex and diet in modeling chemically-induced toxicity. Toxicol Ecotoxicol News 1997; 4: 158–160.

    Google Scholar 

  21. Snell GD. Biology of the Laboratory Mouse, 1st Edn. McGraw-Hill, New York, MA, US, 1941.

    Google Scholar 

  22. Brown SD, Moore MW. The International Mouse Phenotyping Consortium: past and future perspectives on mouse phenotyping. Mamm Genome 2012; 23(9-10): 632–640.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  23. O’Connor JC, Lawson MA, André C, Moreau M, Lestage J, Castanon N, Kelley KW, Dantzer R. Lipopolysaccharide-induced depressive-like behavior is mediated by indoleamine 2,3-dioxygenase activation in mice. Mol Psychiatry 2009; 14(5): 511–522.

    Article  PubMed  Google Scholar 

  24. Zhong SZ, Ge QH, Qu R, Li Q, Ma SP. Paeonol attenuates neurotoxicity and ameliorates cognitive impairment induced by d-galactose in ICR mice. J Neurol Sci 2009; 277(1-2): 58–64.

    Article  CAS  PubMed  Google Scholar 

  25. Song SH, Kim JE, Go J, Koh EK, Sung JE, Lee HA, Choi KM, Kim HD, Jung YS, Kim KS, Hwang D Y. Comparison of the response using ICR mice derived from three different sources to ethanol/hydrochloric acid-induced gastric injury. Lab Anim Res 2016; 32(1): 56–64.

    Article  PubMed  PubMed Central  Google Scholar 

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This study was supported by a grant (14181MFDS622) from Ministry ofFood and Drug Safety in 2014.

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Correspondence to Myeon-Woo Chung.

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Shin, HJ., Cho, Y.M., Shin, H.J. et al. Comparison of commonly used ICR stocks and the characterization of Korl:ICR. Lab Anim Res 33, 8–14 (2017).

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