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Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains
Laboratory Animal Research volume 33, pages 132–139 (2017)
Adaptive immunity is a type of immune response mediated by T and B cells, and is important response for immune response amplification and memory. In this study, the adaptive immunologic properties of C57BL/6NKorl substrain were compared with those of two other C57BL/6N substrains. There were no significant differences between the C57BL/6NKorl and the two other C57BL/6N substrains in the histological structures of the thymus and spleen, which are immunologic organs containing T cell and B cells. In addition, flow cytometric analysis did not reveal any significant differences in the distribution of T and B cell populations of the three substrains. To evaluate cell-mediated immunity of T cells in the three different substrains, we treated isolated T cells from spleen with Con A. The T cells of C57BL/ 6NKorl showed Con A-dependent proliferation of T cells at lower cell number than those in T cells from the other two C57BL/6N substrains. B cell-mediated humoral immune responses were not significant different among the three substrains. Thus, the results of this study provide evidence that C57BL/6NKorl mice are similar to those two other C57BL/6N substrains in humoral immunity, but C57BL/6NKorl has stronger response in cell mediated immunity.
Wade CM, Daly MJ. Genetic variation in laboratory mice. Nat Genet 2005; 37(11): 1175–1180.
Barros SF, Friedlanskaia I. Petricevich VL, Kipnis TL. Local inflammation, lethality and cytokine release in mice injected with Bothrops atrox venom. Mediators Inflamm 1998; 7(5): 339–346.
Crawley JN, Belknap JK, Collins A. Crabbe JC, Frankel W. Henderson N. Hitzemann RJ, Maxson SC, Miner LL, Silva AJ, Wehner JM, Wynshaw-Boris A. Paylor R. Behavioral phenotypes of inbred mouse strains: implications and recommendations for molecular studies. Psychopharmacology (Berl) 1997; 132(2): 107–124.
Harris RB, Mitchell TO, Yan X. Simpson JS, Redmann SM Jr. Metabolic responses to leptin in obese db/db mice are strain dependent. Am J Physiol Regul Integr Comp Physiol 2001; 281(1): R115–132.
Kile BT, Mason-Garrison CL, Justice MJ. Sex and strain-related differences in the peripheral blood cell values of inbred mouse strains. Mamm Genome 2003; 14(1): 81–85.
Madiehe AM, Hebert S. Mitchell TO, Harris RB. Strain-dependent stimulation of growth in leptin-treated obese db/db mice. Endocrinology 2002; 143(10): 3875–3883.
Nishina PM, Wang J. Toyofuku W. Kuypers FA, Ishida BY, Paigen B. Atherosclerosis and plasma and liver lipids in nine inbred strains of mice. Lipids 1993; 28(7): 599–605.
Opsahl ML, McClenaghan M. Springbett A. Reid S. Lathe R. Colman A. Whitelaw CB. Multiple effects of genetic background on variegated transgene expression in mice. Genetics 2002; 160(3): 1107–1112.
Rossmeisl M. Rim JS, Koza RA, Kozak LP. Variation in type 2 diabetes-related traits in mouse strains susceptible to diet-induced obesity. Diabetes 2003; 52(8): 1958–1966.
Sellers RS, Clifford CB, Treuting PM, Brayton C. Immunological variation between inbred laboratory mouse strains: points to consider in phenotyping genetically immunomodified mice. Vet Pathol 2012; 49(1): 32–43.
van Bogaert MJ, Groenink L. Oosting RS, Westphal KG, van der Gugten J. Olivier B. Mouse strain differences in autonomic responses to stress. Genes Brain Behav 2006; 5(2): 139–149.
Woodworth CD, Michael E. Smith L. Vijayachandra K. Glick A. Hennings H. Yuspa SH. Strain-dependent differences in malignant conversion of mouse skin tumors is an inherent property of the epidermal keratinocyte. Carcinogenesis 2004; 25(9): 1771–1778.
Dux A. Muhlbock O. Bailey DW. Genetic analyses of differences in incidence of mammary tumors and reticulum cell neoplasms with the use of recombinant inbred lines of mice. J Natl Cancer Inst 1978; 61(4): 1125–1129.
Festing MF. Properties of inbred strains and outbred stocks, with special reference to toxicity testing. J Toxicol Environ Health 1979; 5(1): 53–68.
Zurita E. Chagoyen M. Cantero M. Alonso R. Gonzalez-Neira A. Lopez-Jimenez A. Lopez-Moreno JA, Landel CP, Benitez J. Pazos F. Montoliu L. Genetic polymorphisms among C57BL/6 mouse inbred strains. Transgenic Res 2011; 20(3): 481–489.
Talmadge JE, Meyers KM, Prieur DJ, Starkey JR. Role of natural killer cells in tumor growth and metastasis: C57BL/6 normal and beige mice. J Natl Cancer Inst 1980, 65(5): 929–935.
Boehm T Design principles of adaptive immune systems. Nat Rev Immunol 2011; 11(5): 307–317.
Chen J. Flurkey K. Harrison DE. A reduced peripheral blood CD4(+) lymphocyte proportion is a consistent ageing phenotype. Mech Ageing Dev 2002; 123(2–3): 145–153.
Chen J. Harrison DE. Quantitative trait loci regulating relative lymphocyte proportions in mouse peripheral blood. Blood 2002; 99(2): 561–566.
Glineur S. Antoine- Moussiaux N. Michaux C. Desmecht D. Immune depression of the SJL/J mouse, a radioresistant and immunologically atypical inbred strain. Immunobiology 2011; 216(1–2): 213–217.
Mayer A. Lilly F. Duran-Reynals ML. Genetically dominant resistance in mice to 3-methylcholanthrene-induced lymphoma. Proc Natl Acad Sci U S A 1980; 77(5): 2960–2963.
McVicar DW, Winkler-Pickett R. Taylor LS, Makrigiannis A. Bennett M. Anderson SK, Ortaldo JR. Aberrant DAP 12 signaling in the 129 strain of mice: implications for the analysis of gene-targeted mice. J Immunol 2002; 169(4): 1721–1728.
Mills CD, Kincaid K. Alt JM, Heilman MJ, Hill AM. M-1/M-2 macrophages and the Th1/Th2 paradigm. J Immunol 2000; 164(12): 6166–6173.
Green MC, Grueneberg H. Hertwig P. Heston WE, Lyon MF, Medvedev NN, Snell GD, Staats J. A revision of the standardized genetic nomenclature for mice. J Hered 1963; 54: 159–162.
Eppig JT, Blake JA, Bult CJ, Kadin JA, Richardson JE; Mouse Genome Database Group. The mouse genome database (MGD): new features facilitating a model system. Nucleic Acids Res 2007; 35(Database issue): D630–637.
Silver LM. Mouse genetics: concepts and applications. New York: Oxford University Press; 1995; pp 3–31.
This project was supported by a grant of BIOREIN (Laboratory Animal Bio Resources Initiative) from Ministry of Food and Drug Safety in 2016.
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Kim, H.R., Choi, J.Y., Kim, K.S. et al. Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains. Lab Anim Res 33, 132–139 (2017). https://doi.org/10.5625/lar.2017.33.2.132
- T cell
- B cell
- cell-mediated immunity
- humoral immunity