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Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains


Adaptive immunity is a type of immune response mediated by T and B cells, and is important response for immune response amplification and memory. In this study, the adaptive immunologic properties of C57BL/6NKorl substrain were compared with those of two other C57BL/6N substrains. There were no significant differences between the C57BL/6NKorl and the two other C57BL/6N substrains in the histological structures of the thymus and spleen, which are immunologic organs containing T cell and B cells. In addition, flow cytometric analysis did not reveal any significant differences in the distribution of T and B cell populations of the three substrains. To evaluate cell-mediated immunity of T cells in the three different substrains, we treated isolated T cells from spleen with Con A. The T cells of C57BL/ 6NKorl showed Con A-dependent proliferation of T cells at lower cell number than those in T cells from the other two C57BL/6N substrains. B cell-mediated humoral immune responses were not significant different among the three substrains. Thus, the results of this study provide evidence that C57BL/6NKorl mice are similar to those two other C57BL/6N substrains in humoral immunity, but C57BL/6NKorl has stronger response in cell mediated immunity.


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This project was supported by a grant of BIOREIN (Laboratory Animal Bio Resources Initiative) from Ministry of Food and Drug Safety in 2016.

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Correspondence to Dae Youn Hwang.

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Kim, H.R., Choi, J.Y., Kim, K.S. et al. Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains. Lab Anim Res 33, 132–139 (2017).

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  • C57BL/6N
  • C57BL/6NKorl
  • T cell
  • B cell
  • cell-mediated immunity
  • humoral immunity