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Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains

Laboratory Animal Research volume 33, pages 132–139 (2017)Cite this article

Abstract

Adaptive immunity is a type of immune response mediated by T and B cells, and is important response for immune response amplification and memory. In this study, the adaptive immunologic properties of C57BL/6NKorl substrain were compared with those of two other C57BL/6N substrains. There were no significant differences between the C57BL/6NKorl and the two other C57BL/6N substrains in the histological structures of the thymus and spleen, which are immunologic organs containing T cell and B cells. In addition, flow cytometric analysis did not reveal any significant differences in the distribution of T and B cell populations of the three substrains. To evaluate cell-mediated immunity of T cells in the three different substrains, we treated isolated T cells from spleen with Con A. The T cells of C57BL/ 6NKorl showed Con A-dependent proliferation of T cells at lower cell number than those in T cells from the other two C57BL/6N substrains. B cell-mediated humoral immune responses were not significant different among the three substrains. Thus, the results of this study provide evidence that C57BL/6NKorl mice are similar to those two other C57BL/6N substrains in humoral immunity, but C57BL/6NKorl has stronger response in cell mediated immunity.

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Acknowledgments

This project was supported by a grant of BIOREIN (Laboratory Animal Bio Resources Initiative) from Ministry of Food and Drug Safety in 2016.

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Authors and Affiliations

  1. Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Korea

    Hye Ryeong Kim, Jun Young Choi & Dae Youn Hwang

  2. College of Veterinary Medicine, Kyungpook National University, Daegu, 41566, Korea

    Kil Soo Kim

  3. College of Pharmacy, Pusan National University, Busan, 46241, Korea

    Young-Suk Jung

  4. Exercise Biochemistry Laboratory, Korea National Sport University, Seoul, 05541, Korea

    Joon Young Cho

  5. Department of Microbiology and Immunology, INJE University College of Medicine, Busan, 47392, Korea

    Hyun Keun Song

  6. Department of Microbiology and Immunology, INJE University College of Medicine, 633-165 CaegumDong, BusanjinGu, Busan, 614-735, Korea

    Hyun Keun Song

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  1. Hye Ryeong Kim
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Correspondence to Dae Youn Hwang.

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Kim, H.R., Choi, J.Y., Kim, K.S. et al. Comparison of humoral and cell-mediated immunity in three different C57BL/6N mouse substrains. Lab Anim Res 33, 132–139 (2017). https://doi.org/10.5625/lar.2017.33.2.132

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  • DOI: https://doi.org/10.5625/lar.2017.33.2.132

Keywords

  • C57BL/6N
  • C57BL/6NKorl
  • T cell
  • B cell
  • cell-mediated immunity
  • humoral immunity

Laboratory Animal Research

ISSN: 2233-7660