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Comparative study of fertilization rates of C57BL/6NKorl and C57BL/6N mice obtained from two other sources

Laboratory Animal Research volume 33, pages 179–186 (2017)Cite this article

Abstract

C57BL/6N is the most widely used inbred mouse strain applied in a wide variety of research areas including cancer, cardiovascular biology, developmental biology, diabetes and obesity, genetics, immunology, neurobiology, and sensorineural research. To compare the fertilization rates of C57BL/6NKorl mice with two commercial C57BL/6N stocks, differences in reproductive organ structures, sperm and egg numbers, fertilization rates, and embryo development rates among C57BL/6NKorl (Korea FDA source), C57BL/6NA (USA source), and C57BL/6NB (Japan source) mice were determined. Among the stocks, no significant differences were detected in organ weight and histological structure of male and female reproductive organs, although body weight was higher in C57BL/6NKorl mice than that in the other groups. The concentration and morphology of sperm and eggs in C57BL/6NKorl mice were similar to those of C57BL/6NA and C57BL/6NB mice. Furthermore, the three stocks had similar in vitro fertilization and embryo development rates, although these rates tended to be higher in C57BL/6NB mice. Pup body weight was higher in C57BL/6NKorl and C57BL/6NB mice than that in C57BL/6NA mice. The results of the present study suggest that C57BL/6NKorl, C57BL/6NA, and C57BL/6NB mice obtained from three different sources have similar fertilization and embryo development rates, although there were slight differences in the magnitude of their responses rates.

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Acknowledgments

We thank Jin Hyang Hwang, the animal technician, for directing the animal care at the Laboratory Animal Resources Center. This project was supported by a 2015 grant from BIOREIN (Laboratory Animal Bio Resources Initiative) of the Ministry of Food and Drug Safety.

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  1. These authors equally contributed to this work.

Authors and Affiliations

  1. Department of Biomaterials Science, College of Natural Resources and Life Science/Life and Industry Convergence Research Institute, Pusan National University, 50 Cheonghak, Samnangjin-eup, Miryang, Gyeongnam, 627-706, Korea

    Woo Bin Yun, Hye Ryeong Kim, Ji Eun Kim, Jun Young Choi, Mi Rim Lee, Jin Ju Park, Bo Ram Song & Dae Youn Hwang

  2. Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, Korea

    Young Suk Jung

  3. Departmentof Microbiology and Immunology, Inje University College of Medicine, Busan, Korea

    Hyun Keun Song

  4. Department of Health and Exercise Science, Korea National Sport University, Seoul, Korea

    Joon Young Cho

  5. College of Veterinary Medicine, Kyungpook National University, Daegu, Korea

    Kil Soo Kim

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  1. Woo Bin Yun
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  2. Hye Ryeong Kim
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  3. Ji Eun Kim
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  8. Young Suk Jung
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  11. Kil Soo Kim
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  12. Dae Youn Hwang
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Correspondence to Dae Youn Hwang.

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Yun, W.B., Kim, H.R., Kim, J.E. et al. Comparative study of fertilization rates of C57BL/6NKorl and C57BL/6N mice obtained from two other sources. Lab Anim Res 33, 179–186 (2017). https://doi.org/10.5625/lar.2017.33.2.179

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  • DOI: https://doi.org/10.5625/lar.2017.33.2.179

Keywords

Laboratory Animal Research

ISSN: 2233-7660