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Quinolone susceptibility and genetic characterization of Salmonella enterica subsp. enterica isolated from pet turtles

Laboratory Animal Research volume 33, pages 49–56 (2017)Cite this article

Abstract

Turtle-borne Salmonella enterica owns significance as a leading cause in human salmonellosis. The current study aimed to determine the quinolone susceptibility and the genetic characteristics of 21 strains of S. enterica subsp. enterica isolated from pet turtles. Susceptibility of four antimicrobials including nalidixic acid, ciprofloxacin, ofloxacin, and levofloxacin was examined in disk diffusion and MIC tests where the majority of the isolates were susceptible to all tested quinolones. In genetic characterization, none of the isolates were positive for qnr or aac(6’)-Ib genes and no any target site mutations could be detected in gyrA, gyrB, and parC quinolone resistance determining regions (QRDR). In addition, neighbor-joining phylogenetic tree derived using gyrA gene sequences exhibited two distinct clads comprising; first, current study isolates, and second, quinolone-resistant isolates of human and animal origin. All results suggest that studied strains of S. enterica subsp. enterica isolated from pet turtles are susceptible to quinolones and genetically more conserved with regards to gyrA gene region.

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Acknowledgments

This study was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2015R1D1A1A01060638).

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Authors and Affiliations

  1. Veterinary Medical Center and College of Veterinary Medicine, Chungbuk National University, Chungdaero 1, Seowon-gu, Cheongju, Chungbuk, 28644, Korea

    B. C. J. De Silva, Sabrina Hossain, S. H. M. P. Wimalasena, H. N. K. S. Pathirana, Mitchell Wendt & Gang-Joon Heo

Authors
  1. B. C. J. De Silva
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  2. Sabrina Hossain
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  3. S. H. M. P. Wimalasena
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  4. H. N. K. S. Pathirana
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  6. Gang-Joon Heo
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Corresponding author

Correspondence to Gang-Joon Heo.

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De Silva, B.C.J., Hossain, S., Wimalasena, S.H.M.P. et al. Quinolone susceptibility and genetic characterization of Salmonella enterica subsp. enterica isolated from pet turtles. Lab Anim Res 33, 49–56 (2017). https://doi.org/10.5625/lar.2017.33.2.49

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  • DOI: https://doi.org/10.5625/lar.2017.33.2.49

Keywords

  • Salmonella enterica subsp. enterica
  • quinolone susceptibility
  • pet turtles
  • qnr genes
  • QRDR

Laboratory Animal Research

ISSN: 2233-7660