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Artemisia argyi attenuates airway inflammation in lipopolysaccharide induced acute lung injury model

Laboratory Animal Research volume 33, pages 209–215 (2017)Cite this article

Abstract

Artemisia argyi is used as a health supplement, tea, and food source in Korea. This study aimed to evaluate the effect of Artemisia argyi (AA) and its active compound, dehydromatricarin A (DA), on the attenuation of airway inflammation in a murine model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). The C57BL/6 mice were administered AA (50 mg/kg or 100 mg/kg) and DA (10 mg/kg or 20 mg/kg) by oral gavage from day 0 to 7 days and LPS treated by intranasal instillation 48 hours before the sacrifice. The treatment of AA and DA markedly decreased inflammatory cells in the bronchoalveolar lavage fluid (BALF) compared with that in ALI-induced mice, which was accompanied by a significant reduction in the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in BALF. Furthermore, the administration of AA and DA clearly decreased inducible nitric oxide synthase (iNOS) expression and nuclear factor kappa B (NF-κB) phosphorylation in comparison with that in the ALI-induced mice. The histological examination of the lung tissue revealed that the administration of AA and DA suppressed the inflammatory cell infiltration into the peribronchial and alveolar lesions induced by LPS instillation. Collectively, our results indicated that AA and DA effectively decreased the airway inflammatory response induced by LPS instillation. Therefore, AA and DA may offer a potential therapy for airway inflammatory disease.

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  1. These authors contributed equally to this work

Authors and Affiliations

  1. College of Veterinary Medicine (BK21 Plus Project Team), Chonnam National University, 77 Yongbongro, Buk-gu, Gwangju, 500-757, Korea

    Na-Rae Shin, Sung-Hyeuk Park, Je-Won Ko, Jong-Choon Kim, Dong-Ho Shin & In-Sik Shin

  2. Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, Chungju, Korea

    Hyung-Won Ryu

  3. Namhae Garlic Research Institute, Namhae-gun, Korea

    Seong-Hun Jeong

  4. Korea Ginseng Corporation, 30 Gajeongro, Yuseong-gu, Daejeon, 305-805, Korea

    Hoon-Sang Lee

Authors
  1. Na-Rae Shin
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  2. Sung-Hyeuk Park
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  3. Je-Won Ko
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  4. Hyung-Won Ryu
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Correspondence to Hoon-Sang Lee or In-Sik Shin.

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Shin, NR., Park, SH., Ko, JW. et al. Artemisia argyi attenuates airway inflammation in lipopolysaccharide induced acute lung injury model. Lab Anim Res 33, 209–215 (2017). https://doi.org/10.5625/lar.2017.33.3.209

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  • DOI: https://doi.org/10.5625/lar.2017.33.3.209

Keywords

  • Artemisia argyi
  • dehydromatricarin A
  • acute lung injury
  • inducible nitric oxide synthase
  • nuclear factor kappa B

Laboratory Animal Research

ISSN: 2233-7660