Skip to main content
Download PDF

Artemisia argyi attenuates airway inflammation in lipopolysaccharide induced acute lung injury model

Laboratory Animal Research volume 33, pages 209–215 (2017)Cite this article

Abstract

Artemisia argyi is used as a health supplement, tea, and food source in Korea. This study aimed to evaluate the effect of Artemisia argyi (AA) and its active compound, dehydromatricarin A (DA), on the attenuation of airway inflammation in a murine model of lipopolysaccharide (LPS)-induced acute lung injury (ALI). The C57BL/6 mice were administered AA (50 mg/kg or 100 mg/kg) and DA (10 mg/kg or 20 mg/kg) by oral gavage from day 0 to 7 days and LPS treated by intranasal instillation 48 hours before the sacrifice. The treatment of AA and DA markedly decreased inflammatory cells in the bronchoalveolar lavage fluid (BALF) compared with that in ALI-induced mice, which was accompanied by a significant reduction in the levels of tumor necrosis factor (TNF)-α and interleukin (IL)-6 in BALF. Furthermore, the administration of AA and DA clearly decreased inducible nitric oxide synthase (iNOS) expression and nuclear factor kappa B (NF-κB) phosphorylation in comparison with that in the ALI-induced mice. The histological examination of the lung tissue revealed that the administration of AA and DA suppressed the inflammatory cell infiltration into the peribronchial and alveolar lesions induced by LPS instillation. Collectively, our results indicated that AA and DA effectively decreased the airway inflammatory response induced by LPS instillation. Therefore, AA and DA may offer a potential therapy for airway inflammatory disease.

References

  1. Mei SH, McCarter SD, Deng Y, Parker CH, Liles WC, Stewart DJ. Prevention of LPS-induced acute lung injury in mice by mesenchymal stem cells overexpressing angiopoietin 1. PLoS Med 2007; 4(9): e269.

    Article  PubMed  PubMed Central  Google Scholar 

  2. Xiao Q, Dong N, Yao X, Wu D, Lu Y, Mao F, Zhu J, Li J, Huang J, Chen A, Huang L, Wang X, Yang G, He G, Xu Y, Lu W. Bufexamac ameliorates LPS-induced acute lung injury in mice by targeting LTA4H. Sci Rep 2016; 6: 25298.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  3. Yang SC, Chang SH, Hsieh PW, Huang YT, Ho CM, Tsai YF, Hwang TL. Dipeptide HCH6-1 inhibits neutrophil activation and protects against acute lung injury by blocking FPR1. Free Radic Biol Med 2017; 106: 254–269.

    Article  CAS  PubMed  Google Scholar 

  4. Pedrazza L, Cunha AA, Luft C, Nunes NK, Schimitz F, Gassen RB, Breda RV, Donadio MV, de Souza Wyse AT, Pitrez PMC, Rosa JL, de Oliveira JR. Mesenchymal stem cells improves survival in LPS-induced acute lung injury acting through inhibition of NETs formation. J Cell Physiol 2017; 232(12): 3552–3564.

    Article  CAS  PubMed  Google Scholar 

  5. Yingkun N, Zhenyu W, Jing L, Xiuyun L, Huimin Y. Stevioside protects LPS-induced acute lung injury in mice. Inflammation 2013; 36(1): 242–250.

    Article  CAS  PubMed  Google Scholar 

  6. Al-Harbi NO, Imam F, Al-Harbi MM, Ansari MA, Zoheir KM, Korashy HM, Sayed-Ahmed MM, Attia SM, Shabanah OA, Ahmad SF. Dexamethasone Attenuates LPS-induced Acute Lung Injury through Inhibition of NF-κB, COX-2, and Pro-inflammatory Mediators. Immunol Invest 2016; 45(4): 349–369.

    Article  CAS  PubMed  Google Scholar 

  7. Wang T, Hou W, Fu Z. Preventative effect of OMZ-SPT on lipopolysaccharide-induced acute lung injury and inflammation via nuclear factor-kappa B signaling in mice. Biochem Biophys Res Commun 2017; 485(2): 284–289.

    Article  CAS  PubMed  Google Scholar 

  8. Wu Y, Jin F, Wang Y, Li F, Wang L, Wang Q, Ren Z, Wang Y. In vitro and in vivo anti-inflammatory effects of theaflavin-3,3’-digallate on lipopolysaccharide-induced inflammation. Eur J Pharmacol 2017; 794: 52–60.

    Article  CAS  PubMed  Google Scholar 

  9. Zhang B, Liu ZY, Li YY, Luo Y, Liu ML, Dong HY, Wang YX, Liu Y, Zhao PT, Jin FG, Li ZC. Antiinflammatory effects of matrine in LPS-induced acute lung injury in mice. Eur J Pharm Sci 2011; 44(5): 573–579.

    Article  CAS  PubMed  Google Scholar 

  10. Yang S, Yu Z, Wang L, Yuan T, Wang X, Zhang X, Wang J, Lv Y, Du G. The natural product bergenin ameliorates lipopolysaccharide-induced acute lung injury by inhibiting NF-kappaB activition. J Ethnopharmacol 2017; 200: 147–155.

    Article  CAS  PubMed  Google Scholar 

  11. Shen B, Zhao C, Chen C, Li Z, Li Y, Tian Y, Feng H. Picroside II Protects Rat Lung and A549 Cell Against LPS-Induced Inflammation by the NF-κB Pathway. Inflammation 2017; 40(3): 752–761.

    Article  CAS  PubMed  Google Scholar 

  12. Li N, Song Y, Zhao W, Han T, Lin S, Ramirez O, Liang L. Small interfering RNA targeting NF-κB attenuates lipopolysaccharide-induced acute lung injury in rats. BMC Physiol 2016; 16(1): 7.

    Article  PubMed  PubMed Central  Google Scholar 

  13. Shin NR, Shin IS, Song HH, Hong JM, Kwon OK, Jeon CM, Kim JH, Lee SW, Lee JK, Jin H, Li WY, Oh SR, Hahn KW, Ahn KS. Callicarpa japonica Thunb. reduces inflammatory responses: a mouse model of lipopolysaccharide-induced acute lung injury. Int Immunopharmacol 2015; 26(1): 174–180.

    Article  CAS  PubMed  Google Scholar 

  14. Kim JK, Shin EC, Lim HJ, Choi SJ, Kim CR, Suh SH, Kim CJ, Park GG, Park CS, Kim HK, Choi JH, Song SW, Shin DH. Characterization of Nutritional Composition, Antioxidative Capacity, and Sensory Attributes of Seomae Mugwort, a Native Korean Variety of Artemisia argyi H. Lév. & Vaniot. J Anal Methods Chem 2015; 2015: 916346.

    PubMed  PubMed Central  Google Scholar 

  15. Yun C, Jung Y, Chun W, Yang B, Ryu J, Lim C, Kim JH, Kim H, Cho SI. Anti-Inflammatory Effects of Artemisia Leaf Extract in Mice with Contact Dermatitis In Vitro and In Vivo. Mediators Inflamm 2016; 2016: 8027537.

    Article  PubMed  PubMed Central  Google Scholar 

  16. Lee NY, Chung KS, Jin JS, Bang KS, Eom YJ, Hong CH, Nugroho A, Park HJ, An HJ. Effect of Chicoric Acid on Mast Cell-Mediated Allergic Inflammation in Vitro and in Vivo. J Nat Prod 2015; 78(12): 2956–2962.

    Article  CAS  PubMed  Google Scholar 

  17. Yao X, Wu D, Dong N, Ouyang P, Pu J, Hu Q, Wang J, Lu W, Huang J. Moracin C. A Phenolic Compound Isolated from Artocarpus heterophyllus, Suppresses Lipopolysaccharide-Activated Inflammatory Responses in Murine Raw264.7 Macrophages. Int J Mol Sci 2016; 17(8).

    Google Scholar 

  18. Shin NR, Ryu HW, Ko JW, Park SH, Yuk HJ, Kim HJ, Kim JC, Jeong SH, Shin IS. Artemisia argyi attenuates airway inflammation in ovalbumin-induced asthmatic animals. J Ethnopharmacol 2017; 209: 108–115.

    Article  PubMed  Google Scholar 

  19. Seehase S, Lauenstein HD, Schlumbohm C, Switalla S, Neuhaus V, Förster C, Fieguth HG, Pfennig O, Fuchs E, Kaup FJ, Bleyer M, Hohlfeld JM, Braun A, Sewald K, Knauf S. LPS-induced lung inflammation in marmoset monkeys-an acute model for anti-inflammatory drug testing. PLoS One 2012; 7(8): e43709.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Yuan X, Wang Y, Du D, Hu Z, Xu M, Xu M, Liu Z. The effects of the combination of sodium ferulate and oxymatrine on lipopolysaccharide-induced acute lung injury in mice. Inflammation 2012; 35(3): 1161–1168.

    Article  CAS  PubMed  Google Scholar 

  21. Kang P, Kim KY, Lee HS, Min SS, Seol GH. Anti-inflammatory effects of anethole in lipopolysaccharide-induced acute lung injury in mice. Life Sci 2013; 93(24): 955–961.

    Article  CAS  PubMed  Google Scholar 

  22. Han HJ, Li M, Son JK, Seo CS, Song SW, Kwak SH, Bae HB. Sauchinone, a lignan from Saururus chinensis, attenuates neutrophil pro-inflammatory activity and acute lung injury. Int Immunopharmacol 2013; 17(2): 471–477.

    Article  CAS  PubMed  Google Scholar 

  23. Niu X, Hu H, Li W, Li Y, Huang H, Mu Q, Yao H, Li H. Protective effect of total alkaloids on lipopolysaccharide-induced acute lung injury. J Surg Res 2014; 189(1): 126–134.

    Article  CAS  PubMed  Google Scholar 

  24. Park HJ, Jeon BT, Kim HC, Roh GS, Shin JH, Sung NJ, Han J, Kang D. Aged red garlic extract reduces lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages and acute pulmonary inflammation through haeme oxygenase-1 induction. Acta Physiol (Oxf) 2012; 205(1): 61–70.

    Article  CAS  Google Scholar 

  25. Sun Q, Chen L, Gao M, Jiang W, Shao F, Li J, Wang J, Kou J, Yu B. Ruscogenin inhibits lipopolysaccharide-induced acute lung injury in mice: involvement of tissue factor, inducible NO synthase and nuclear factor (NF)-κB. Int Immunopharmacol 2012; 12(1): 88–93.

    Article  CAS  PubMed  Google Scholar 

  26. Zhang X, Huang H, Yang T, Ye Y, Shan J, Yin Z, Luo L. Chlorogenic acid protects mice against lipopolysaccharide-induced acute lung injury. Injury 2010; 41(7): 746–752.

    Article  PubMed  Google Scholar 

  27. Wu Q, Sun G, Yuan X, Soromou LW, Chen N, Xiong Y, Feng H. Tubeimoside-1 attenuates LPS-induced inflammation in RAW 264.7 macrophages and mouse models. Immunopharmacol Immunotoxicol 2013; 35(4): 514–523.

    Article  CAS  PubMed  Google Scholar 

  28. Gao M, Chen L, Yu H, Sun Q, Kou J, Yu B. Diosgenin down-regulates NF-κB p65/p50 and p38MAPK pathways and attenuates acute lung injury induced by lipopolysaccharide in mice. Int Immunopharmacol 2013; 15(2): 240–245.

    Article  CAS  PubMed  Google Scholar 

  29. Chi G, Wei M, Xie X, Soromou LW, Liu F, Zhao S. Suppression of MAPK and NF-κB pathways by limonene contributes to attenuation of lipopolysaccharide-induced inflammatory responses in acute lung injury. Inflammation 2013; 36(2): 501–511.

    Article  CAS  PubMed  Google Scholar 

  30. Chen L, Zhao L, Zhang C, Lan Z. Protective effect of p-cymene on lipopolysaccharide-induced acute lung injury in mice. Inflammation 2014; 37(2): 358–364.

    Article  CAS  PubMed  Google Scholar 

  31. Ji MH, Li GM, Jia M, Zhu SH, Gao DP, Fan YX, Wu J, Yang JJ. Valproic acid attenuates lipopolysaccharide-induced acute lung injury in mice. Inflammation 2013; 36(6): 1453–1459.

    Article  CAS  PubMed  Google Scholar 

  32. Huang GJ, Deng JS, Chen CC, Huang CJ, Sung PJ, Huang SS, Kuo YH. Methanol extract of Antrodia camphorata protects against lipopolysaccharide-induced acute lung injury by suppressing NF-κB and MAPK pathways in mice. J Agric Food Chem 2014; 62(23): 5321–5329.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Author notes

  1. These authors contributed equally to this work

Authors and Affiliations

  1. College of Veterinary Medicine (BK21 Plus Project Team), Chonnam National University, 77 Yongbongro, Buk-gu, Gwangju, 500-757, Korea

    Na-Rae Shin, Sung-Hyeuk Park, Je-Won Ko, Jong-Choon Kim, Dong-Ho Shin & In-Sik Shin

  2. Natural Medicine Research Center, Korea Research Institute of Bioscience & Biotechnology, Chungju, Korea

    Hyung-Won Ryu

  3. Namhae Garlic Research Institute, Namhae-gun, Korea

    Seong-Hun Jeong

  4. Korea Ginseng Corporation, 30 Gajeongro, Yuseong-gu, Daejeon, 305-805, Korea

    Hoon-Sang Lee

Authors
  1. Na-Rae Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Sung-Hyeuk Park
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Je-Won Ko
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Hyung-Won Ryu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Seong-Hun Jeong
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jong-Choon Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Dong-Ho Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Hoon-Sang Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. In-Sik Shin
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Hoon-Sang Lee or In-Sik Shin.

Rights and permissions

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://doi.org/creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Shin, NR., Park, SH., Ko, JW. et al. Artemisia argyi attenuates airway inflammation in lipopolysaccharide induced acute lung injury model. Lab Anim Res 33, 209–215 (2017). https://doi.org/10.5625/lar.2017.33.3.209

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.5625/lar.2017.33.3.209

Keywords

Laboratory Animal Research

ISSN: 2233-7660