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Evaluation of in vitro and in vivo genotoxicity of Angelica acutiloba in a standard battery of assays

Laboratory Animal Research volume 33, pages 231–236 (2017)Cite this article

Abstract

Among three representative species of Angelica found in Asian countries, including Korea, China, and Japan, Angelica acutiloba (AA) has been used as traditional herbal medicine with antitumor, antiinflammatory, anti-obesity, and anti-diabetes activities. In this study, the potential genotoxicity and mutagenicity of the AA extract were examined in a battery of in vitro and in vivo tests (bacterial reverse mutation assay, in vitro chromosomal aberrations assay, and in vivo micronucleus assay) in accordance with the test guidelines for toxicity testing developed by the Organization for Economic Cooperation and Development. Upon testing in the bacterial mutation assay (Ames test) using five Salmonella typhimurium TA98, TA100, TA102, TA1535 and TA1537, no significant increase the number of revertant colonies in the metabolic activation system and non-activation system was noted in the AA extract groups. Also, in the chromosome aberration test, the AA extract did not cause chromosomal aberration with or without metabolic activation by S9 mix. A bone marrow micronucleus test of mice demonstrated that the incidence of micronucleated polychromatic erythrocytes in the AA extract groups (500, 1000 and 2000 mg/kg BW) was equivalent to that of the negative control group. Based on these results from a standard battery of assays, the AA extract was concluded to have no genotoxic at the proper dose.

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Authors and Affiliations

  1. Department of Biotechnology, The Catholic University of Korea, Bucheon, Gyeonggi-do, Korea

    Jun-Won Yun

  2. Department of Experimental Animal Research, Biomedical Research Institute, Seoul National University Hospital, Seoul, Korea

    Yun-Soon Kim, Euna Kwon, Seung-Hyun Kim, Ji-Ran You & Byeong-Cheol Kang

  3. Department of Urology, Seoul National University College of Medicine, Seoul, Korea

    Hyeon Hoe Kim

  4. Biomedical Center for Animal Resource and Development, Seoul National University College of Medicine, 103 Daehakro, Jongno-gu, Seoul, 03080, Korea

    Jeong-Hwan Che & Byeong-Cheol Kang

  5. Graduate School of Translational Medicine, Seoul National University College of Medicine, 101 Daehakro, Jongno-gu, Seoul, 110-744, Korea

    Byeong-Cheol Kang

  6. Designed Animal and Transplantation Research Institute, Institute of GreenBio Science Technology, Seoul National University, Pyeongchang-gun, Gangwon-do, Korea

    Byeong-Cheol Kang

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  1. Jun-Won Yun
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  2. Yun-Soon Kim
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Correspondence to Jeong-Hwan Che or Byeong-Cheol Kang.

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Yun, JW., Kim, YS., Kwon, E. et al. Evaluation of in vitro and in vivo genotoxicity of Angelica acutiloba in a standard battery of assays. Lab Anim Res 33, 231–236 (2017). https://doi.org/10.5625/lar.2017.33.3.231

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  • DOI: https://doi.org/10.5625/lar.2017.33.3.231

Keywords

  • Angelica acutiloba
  • traditional medicine
  • genotoxicity
  • mutagenicity

Laboratory Animal Research

ISSN: 2233-7660