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Lipolytic effect of novel extracts from mulberry (Morus alba) leaves fermented with Cordyceps militaris in the primary adipocytes derived from SD rats

Laboratory Animal Research volume 33, pages 270–279 (2017)Cite this article

Abstract

Mulberry (Morus alba) leaves are known to have therapeutic effects on lipid metabolism including lipogenesis, lipolysis and hyperlipidemia. However, novel compounds with strong lipolytic ability among 27 extracts of the mulberry leaves fermented with Cordyceps militaris (EMfCs) have not yet been identified. Therefore, the cAMP concentration and cell viability were measured in the primary adipocytes of SD (Sprague Dawley) rats and 3T3-L1 cells after treatment of 27 EMfCs. Briefly, mulberry leaves powders amended with three different concentrations (0, 25 and 50%) of silkworm pupae (SWP) powder were fermented with 10% C. militaris (v/w) during three different periods (3, 4 and 6 weeks). A total of 27 extracts were obtained from the fermented mulberry leaves powders using three different solvents (dH2O, 50% EtOH and 95% EtOH). Among the 27 EMfCs treated groups, a significant increase in the concentration of cAMP was detected in primary adipocytes treated with 10 extracts when compared with the Vehicle treated group. However, their cAMP concentration did not agree completely with the non-toxicity, although most extracts showed non-toxicity. Furthermore, the concentration of cAMP and level of free glycerol gradually increased in a dose dependent manner (100, 200 and 400μg/mL) of 4M3-95 contained cordycepin without any significant toxicity. Overall, the results of this study provide strong evidence that 4M3-95 extract derived from EMfCs can stimulate the lipolysis of primary adipocytes at an appropriate concentration and therefore have the potential for use as lipolytic agents to treat obesity.

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  1. These authors contributed equally to this work.

Authors and Affiliations

  1. Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Korea

    Mi Rim Lee, Ji Eun Kim, Woo Bin Yun, Jun Young Choi, Jin Ju Park, Hye Ryeong Kim, Bo Ram Song & Dae Youn Hwang

  2. Department of Horticultural Bioscience, College of Natural Resources & Life Science/Life and Industry Convergence Research Institue, Pusan National University, Miryang, 50463, Korea

    Young Whan Choi

  3. Life Science Research Institute, Novarex Co., Ltd., Chungju, 28126, Korea

    Kyung Mi Kim

  4. Department of Biomaterials Science, College of Natural Resources and Life Science, Pusan National University, 50 Cheonghak-ri, Samnangjin-eup Miryang, Gyeongnam, 627-706, Korea

    Dae Youn Hwang

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  1. Mi Rim Lee
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  2. Ji Eun Kim
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  3. Woo Bin Yun
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Correspondence to Dae Youn Hwang.

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Lee, M.R., Kim, J.E., Yun, W.B. et al. Lipolytic effect of novel extracts from mulberry (Morus alba) leaves fermented with Cordyceps militaris in the primary adipocytes derived from SD rats. Lab Anim Res 33, 270–279 (2017). https://doi.org/10.5625/lar.2017.33.3.270

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  • DOI: https://doi.org/10.5625/lar.2017.33.3.270

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Laboratory Animal Research

ISSN: 2233-7660