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Super pregnancy in a BALB/c mouse superovulated with PMSG

Abstract

This paper reports a case of super pregnancy in a BALB/c mouse pregnant with 30 pups following induction of superovulation using a PMSG-hCG protocol. Superovulation was induced in 10 mice by injecting 5 IU PMSG followed by 5 IU hCG 48 hours later. Immediately after injection of hCG, animals were placed with males at a ratio of 1 to 1 for 24 hours. On day 14 after mating, animals were killed by cervical dislocation and the uterus was examined for pregnancy and the number of fetuses. The mean (±SEM) number of fetuses observed in ten mice was 5.4±3.18 with an unexpectedly super pregnant mouse bearing 30 fetuses on day 14 of pregnancy.

References

  1. Rugh R. The mouse. Its reproduction and development. Burgess Pub. Co.; 1968: 225–235.

    Google Scholar 

  2. Luo C, Zuñiga J, Edison E, Palla S, Dong W, Parker-Thornburg J. Superovulation strategies for 6 commonly used mouse strains. J Am Assoc Lab Anim Sci 2011; 50(4): 471–478.

    CAS  PubMed  PubMed Central  Google Scholar 

  3. EDWARDS RG, FOWLER RE. Fetal mortality in adult mice after superovulation with gonadotrophins. J Exp Zool 1959; 141: 299–322.

    Article  CAS  Google Scholar 

  4. Van der Auwera I, D’Hooghe T. Superovulation of female mice delays embryonic and fetal development. Hum Reprod 2001; 16(6): 1237–1243.

    Article  Google Scholar 

  5. Sato A. Some observations on induced polyovulation and superpregnancy in mature mice. Embryologia 1963; 7: 285–294.

    Article  Google Scholar 

  6. Hasegawa A, Mochida K, Inoue H, Noda Y, Endo T, Watanabe G, Ogura A. High-Yield Superovulation in Adult Mice by Anti-Inhibin Serum Treatment Combined with Estrous Cycle Synchronization. Biol Reprod 2016; 94(1): 21.

    Article  Google Scholar 

  7. Corbin TJ, McCabe J G. Strain variation of immature female rats in response to various superovulatory hormone preparations and routes of administration. Contemp Top Lab Anim Sci 2002; 41(2): 18–23.

    CAS  PubMed  Google Scholar 

  8. Behringer R, Gertsenstein M, Nagy K, Nagy A. Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory Press; 2013: 345–360.

    Google Scholar 

  9. Johnson AD. Limitation of fetus number in the rat, mouse and rabbit. J Anim Sci 1970; 30(6): 978–983.

    Article  CAS  Google Scholar 

  10. Ohta H, Sakaide Y, Wakayama T. Age- and substrain-dependent sperm abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI. Hum Reprod 2009; 24(4): 775–781.

    Article  Google Scholar 

  11. Ozgunen KT, Erdogan S, Mazmanoglu N, Pamuk I, Logoglu G, Ozgunen T. Effect of gonadotrophin dose on oocyte retrieval in superovulated BALB/c mice. Theriogenology 2001; 56(3): 435–445.

    Article  CAS  Google Scholar 

  12. Mauleon P. Oogenesis and Folliculogenesis. In: Reproduction in Domestic Animals (Second Edition): Academic Press; 1969: 187–215.

    Chapter  Google Scholar 

  13. Pontes JH, Melo Sterza FA, Basso AC, Ferreira CR, Sanches BV, Rubin KC, Seneda MM. Ovum pick up, in vitro embryo production, and pregnancy rates from a large-scale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology 2011; 75(9): 1640–1646.

    Article  CAS  Google Scholar 

  14. Loutradis D, Drakakis P, Vomvolaki E, Antsaklis A. Different ovarian stimulation protocols for women with diminished ovarian reserve. J Assist Reprod Genet 2007; 24(12): 597–611.

    Article  CAS  Google Scholar 

  15. Lubritz DL, Eisen EJ, Robison OW. Effect of selection for litter size and body weight on hormone-induced ovulation rate in mice. J Anim Sci 1991; 69(11): 4299–4305.

    Article  CAS  Google Scholar 

  16. Spearow JL, Barkley M. Genetic control of hormone-induced ovulation rate in mice. Biol Reprod 1999; 61(4): 851–856.

    Article  CAS  Google Scholar 

  17. Argente MJ, Santacreu MA, Climent A, Blasco A. Influence of available uterine space per fetus on fetal development and prenatal survival in rabbits selected for uterine capacity. Livestock Science 2006; 102: 83–91.

    Article  Google Scholar 

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Correspondence to Asghar Mogheiseh.

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Vaseghi, H., Mogheiseh, A., Sepehrimanesh, M. et al. Super pregnancy in a BALB/c mouse superovulated with PMSG. Lab Anim Res 33, 280–282 (2017). https://doi.org/10.5625/lar.2017.33.3.280

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  • DOI: https://doi.org/10.5625/lar.2017.33.3.280

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