Super pregnancy in a BALB/c mouse superovulated with PMSG
Laboratory Animal Research volume 33, pages 280–282 (2017)
This paper reports a case of super pregnancy in a BALB/c mouse pregnant with 30 pups following induction of superovulation using a PMSG-hCG protocol. Superovulation was induced in 10 mice by injecting 5 IU PMSG followed by 5 IU hCG 48 hours later. Immediately after injection of hCG, animals were placed with males at a ratio of 1 to 1 for 24 hours. On day 14 after mating, animals were killed by cervical dislocation and the uterus was examined for pregnancy and the number of fetuses. The mean (±SEM) number of fetuses observed in ten mice was 5.4±3.18 with an unexpectedly super pregnant mouse bearing 30 fetuses on day 14 of pregnancy.
Rugh R. The mouse. Its reproduction and development. Burgess Pub. Co.; 1968: 225–235.
Luo C, Zuñiga J, Edison E, Palla S, Dong W, Parker-Thornburg J. Superovulation strategies for 6 commonly used mouse strains. J Am Assoc Lab Anim Sci 2011; 50(4): 471–478.
EDWARDS RG, FOWLER RE. Fetal mortality in adult mice after superovulation with gonadotrophins. J Exp Zool 1959; 141: 299–322.
Van der Auwera I, D’Hooghe T. Superovulation of female mice delays embryonic and fetal development. Hum Reprod 2001; 16(6): 1237–1243.
Sato A. Some observations on induced polyovulation and superpregnancy in mature mice. Embryologia 1963; 7: 285–294.
Hasegawa A, Mochida K, Inoue H, Noda Y, Endo T, Watanabe G, Ogura A. High-Yield Superovulation in Adult Mice by Anti-Inhibin Serum Treatment Combined with Estrous Cycle Synchronization. Biol Reprod 2016; 94(1): 21.
Corbin TJ, McCabe J G. Strain variation of immature female rats in response to various superovulatory hormone preparations and routes of administration. Contemp Top Lab Anim Sci 2002; 41(2): 18–23.
Behringer R, Gertsenstein M, Nagy K, Nagy A. Manipulating the mouse embryo: a laboratory manual. Cold Spring Harbor Laboratory Press; 2013: 345–360.
Johnson AD. Limitation of fetus number in the rat, mouse and rabbit. J Anim Sci 1970; 30(6): 978–983.
Ohta H, Sakaide Y, Wakayama T. Age- and substrain-dependent sperm abnormalities in BALB/c mice and functional assessment of abnormal sperm by ICSI. Hum Reprod 2009; 24(4): 775–781.
Ozgunen KT, Erdogan S, Mazmanoglu N, Pamuk I, Logoglu G, Ozgunen T. Effect of gonadotrophin dose on oocyte retrieval in superovulated BALB/c mice. Theriogenology 2001; 56(3): 435–445.
Mauleon P. Oogenesis and Folliculogenesis. In: Reproduction in Domestic Animals (Second Edition): Academic Press; 1969: 187–215.
Pontes JH, Melo Sterza FA, Basso AC, Ferreira CR, Sanches BV, Rubin KC, Seneda MM. Ovum pick up, in vitro embryo production, and pregnancy rates from a large-scale commercial program using Nelore cattle (Bos indicus) donors. Theriogenology 2011; 75(9): 1640–1646.
Loutradis D, Drakakis P, Vomvolaki E, Antsaklis A. Different ovarian stimulation protocols for women with diminished ovarian reserve. J Assist Reprod Genet 2007; 24(12): 597–611.
Lubritz DL, Eisen EJ, Robison OW. Effect of selection for litter size and body weight on hormone-induced ovulation rate in mice. J Anim Sci 1991; 69(11): 4299–4305.
Spearow JL, Barkley M. Genetic control of hormone-induced ovulation rate in mice. Biol Reprod 1999; 61(4): 851–856.
Argente MJ, Santacreu MA, Climent A, Blasco A. Influence of available uterine space per fetus on fetal development and prenatal survival in rabbits selected for uterine capacity. Livestock Science 2006; 102: 83–91.
About this article
Cite this article
Vaseghi, H., Mogheiseh, A., Sepehrimanesh, M. et al. Super pregnancy in a BALB/c mouse superovulated with PMSG. Lab Anim Res 33, 280–282 (2017). https://doi.org/10.5625/lar.2017.33.3.280
- Super pregnancy