Protective effects of cultured and fermented ginseng extracts against scopolamine-induced memory loss in a mouse model
Laboratory Animal Research volume 34, pages 37–43 (2018)
This study was performed to investigate the effect of a concentrate of fermented wild ginseng root culture (HLJC0701) on memory improvement in the scopolamine (SPL)-induced memory-deficient mouse model. Eight-week-old male ICR mice were used to evaluate the protective effect of HLJC0701 against the SPL-induced memory loss animal model. The Morris water maze test, which measures hippocampus-dependent learning ability, and the Y-maze test, a short-term memory assessment test, were performed and related markers were analyzed. HLJG0701-treated groups displayed significantly reduced acetylcholinesterase activity and increased acetylcholine level compared with the SPL-administered group (SPL-G) (P<0.05). In the Y-maze test, the spontaneous alternation in al HLJC0711-treated groups was significantly increased compared with that in SPL-G (P<0.05). In the Morris water maze test, the escape latency and time spent in the target quadrant in all HLJC0701-treated groups were significantly decreased and increased, respectively, compared with those in SPL-G (P<0.05). In addition, the brain-derived neurotrophic factor level in groups treated with HLJC0701 300 and 600 mg/kg body weight was significantly increased compared with that in SPL-G (P<0.05). These results suggest that the HLJC0701 may protect against memory loss by inhibiting acetylcholinesterase activity and preventing acetylcholine deficiency.
Monti JM, Baym CL, Cohen NJ. Identifying and characterizing the effects of nutrition on hippocampal memory. Adv Nutr 2014; 5(3): 337S–343S.
Oh SK. Neurotransmitters and brain disease. Shinil Books Company, Seoul, 2005; pp 345–364.
Fratiglioni L, Winblad B, von Strauss E. Prevention of Alzheimer’s disease and dementia. Major findings from the Kungsholmen Project. Physiol Behav 2007; 92(1-2): 98–104.
Bohnen NI, Albin RL. The cholinergic system and Parkinson disease. Behav Brain Res 2011; 221(2): 564–573.
Schliebs R, Arendt T. The cholinergic system in aging and neuronal degeneration. Behav Brain Res 2011; 221(2): 555–563.
Klinkenberg I, Blokland A. The validity of scopolamine as a pharmacological model for cognitive impairment: a review of animal behavioral studies. Neurosci Biobehav Rev 2010; 34(8): 1307–1350.
Oh JH, Choi BJ, Chang MS, Park SK. Nelumbo nucifera semen extract improves memory in rats with scopolamine-induced amnesia through the induction of choline acetyltransferase expression. Neurosci Lett 2009; 461(1): 41–44.
Han JY, Chung KH, Ryu GH. Comparison of physicochemical properties and release characteristics of extruded tissue cultured mountain ginseng. J Korean Soc Food Sci Nutr 2008; 37(8): 1018–1024.
Zhu G, Wang Y, Li J, Wang J. Chronic treatment with ginsenoside Rgl promotes memory and hippocampal long-term potentiation in middle-aged mice. Neuroscience 2015; 292: 81–89.
Niu J, Pi ZF, Yue H, Yang H, Wang Y, Yu Q, Liu SY. Effect of 20(S)-ginsenoside Rg3 on streptozotocin-induced experimental type 2 diabetic rats: A urinary metabonomics study by rapidresolution liquid chromatography/mass spectrometry. Rapid Commun Mass Spectrom 2012; 26(23): 2683–2689.
Leung KW, Wong AS. Pharmacology of ginsenosides: a literature review. Chin Med 2010; 5: 20.
Bai Y, Gänzle MG. Conversion of ginsenosides by Lactobacillus plantarum studied by liquid chromatography coupled to quadrupole trap mass spectrometry. Food Res Int 2015; 76(Pt 3): 709–718.
Shin EJ, Cho CW, Kim YE, Han D, Hong HD, Rhee YK. Evaluation of functional properties of the tissue cultured wild ginseng fermented by Lactobacillus sp. Korean J Food Culture 2012; 27(6): 743–750.
Panza F, Lozupone M, Solfrizzi V, Stallone R, Bellomo A, Greco A, Daniele A, Seripa D, Logroscino G Cognitive frailty: a potential target for secondary prevention of dementia. Expert Opin Drug Metab Toxicol 2017; 13(10): 1023–1027.
Rakesh G, Szabo ST, Alexopoulos GS, Zannas AS. Strategies for dementia prevention: latest evidence and implications. Ther Adv Chronic Dis 2017; 8(8-9): 121–104.
Wittstock M, Zettl UK. Adverse effects of treatment with intravenous immunoglobulins for neurological diseases. J Neurol 2006; 253 Suppl 5: V75–79.
Farlow M, Gracon SI, Hershey LA, Lewis KW, Sadowsky CH, Dolan-Ureno J. A controlled trial of tacrine in Alzheimer’s disease. JAMA 1992; 268(18): 2523–2529.
Morris R. Developments of a water-maze procedure for studying spatial learning in the rat. J Neurosci Methods 1984; 11(1): 47–60.
Kim DH, Jeon SJ, Son KH, Jung JW, Lee S, Yoon BH, Lee JJ, Cho YW, Cheong JH, Ko KH, Ryu JH. The ameliorating effect of oroxylin A on scopolamine-induced memory impairment in mice. Neurobiol Learn Mem 2007; 87(4): 536–546.
Jahn H. Memory loss in Alzheimer’s disease. Dialogues Clin Neurosci 2013; 15(4): 445–454.
Terry AV Jr, Mahadik SP. Time-dependent cognitive deficits associated with first and second generation antipsychotics: cholinergic dysregulation as a potential mechanism. J Pharmacol Exp Ther 2007; 320(3): 961–968.
Remya C, Dileep KV, Tintu I, Variyar EJ, Sadasivan C. Flavanone glycosides as acetylcholinesterase inhibitors: computational and experimental evidence. Indian J Pharm Sci 2014; 76(6): 567–570.
Colović MB, Krstić DZ, Lazarević-Pašti TD, Bondžić AM, Vasiae VM. Acetylcholinesterase inhibitors: pharmacology and toxicology. Curr Neuropharmacol 2013; 11(3): 315–335.
Wang Q, Sun LH, Jia W, Liu XM, Dang HX, Mai WL, Wang N, Steinmetz A, Wang YQ, Xu CL. Comparison of ginsenosides Rgl and Rbl for their effects on improving scopolamine-induced learning and memory impairment in mice. Phytother Res 2010; 24(12): 1748–1754.
Kim J, Shim J, Lee S, Cho WH, Hong E, Lee JH, Han JS, Lee HJ, Lee KW. Rg3-enriched ginseng extract ameliorates scopolamine-induced learning deficits in mice. BMC Complement Altern Med 2016; 16: 66.
Al-Hazmi MA, Rawi SM, Arafa NM, Wagas A, Montasser AO. The potent effects of ginseng root extract and memantine on cognitive dysfunction in male albino rats. Toxicol Ind Health 2015; 31(6): 494–509.
Jeong HS, Lim CS, Cha BC, Choi SH, Kwon KR. Component analysis of cultivated ginseng, cultivated wild ginseng, and wild ginseng and the change of ginsenoside components in the process of red ginseng. J Pharmacopuncture 2010; 13(1): 63–77.
Nagahara AH, Merrill DA, Coppola G, Tsukada S, Schroeder BE, Shaked GM, Wang L, Blesch A, Kim A, Conner JM, Rockenstein E, Chao MV, Koo EH, Geschwind D, Masliah E, Chiba AA, Tuszynski MH. Neuroprotective effects of brain-derived neurotrophic factor in rodent and primate models of Alzheimer’s disease. Nat Med 2009; 15(3): 331–337.
Konar A, Shah N, Singh R, Saxena N, Kaul SC, Wadhwa R, Thakur MK. Protective role of Ashwagandha leaf extract and its component withanone on scopolamine-induced changes in the brain and brain-derived cells. PLoS One 2011; 6(11): e27265.
Zhao HF, Li Q, Li Y. Long-term ginsenoside administration prevents memory loss in aged female C57BL/6J mice by modulating the redox status and up-regulating the plasticityrelated proteins in hippocampus. Neuroscience 2011; 183: 189–202.
Lee Y, Oh S. Administration of red ginseng ameliorates memory decline in aged mice. J Ginseng Res 2015; 39(3): 250–256.
Dela Peña III, Kim HJ, Botanas CJ, de la Peña JB, Van Le TH, Nguyen MD, Park JH, Cheong JH. The psychopharmacological activities of Vietnamese ginseng in mice: characterization of its psychomotor, sedative-hypnotic, antistress, anxiolytic, and cognitive effects. J Ginseng Res 2017; 41(2): 201–208.
Peña ID, Yoon SY, Kim HJ, Park S, Hong EY, Ryu JH, Park IH, Cheong JH. Effects of ginseol k-g3, an Rg3-enriched fraction, on scopolamine-induced memory impairment and learning deficit in mice. J Ginseng Res 2014; 38(1): 1–7.
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Han, SH., Kim, SJ., Yun, Y.W. et al. Protective effects of cultured and fermented ginseng extracts against scopolamine-induced memory loss in a mouse model. Lab Anim Res 34, 37–43 (2018). https://doi.org/10.5625/lar.2018.34.1.37
- Memory loss