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Anti-Helicobacter pylori activity and inhibition of gastritis by Allium hookeri extract

Laboratory Animal Research volume 34, pages 75–79 (2018)Cite this article

Abstract

Allium hookeri is widely consumed plant as a vegetable and herbal medicine in southeastern Asia. Allium hookeri has been reported antioxidant, improvement of bone health and antidiabetic effects. In the present study, we investigated the potential inhibitory effect of Allium hookeri extract (AHE) on Helicobacter pylori. The in vitro anti-bacterial activities of AHE were determined by disk agar diffusion method. Also, the inhibition effect of the AHE on H. pylori infection was investigated using a mouse model. H. pylori colonization was confirmed by rapid urease tests, as described previously. Mucosal damage was evaluated grossly and histologically according to previously described criteria. As the results of the disk agar diffusion assay, CLR, AMX and MTZ inhibited the bacterial growth with inhibition zone of 19.2, 15.2 and 7.5 mm, respectively. AHE 100 ώg/mL showed an inhibition zone value of 20.6 mm. Rapid urease tests of the mice stomachs demonstrated a significant reduction in H. pylori colonization. In addition to the therapeutic effect against H. pylori infection, the AHE reduced mucosal inflammation and epithelial damages in the stomach of H. pylori-infected mice. These results demonstrate that the AHE successfully cured an H. pylori infection and treated the H. pylori infection. This AHE could be a promising treatment for patients with gastric complaints including gastritis caused by H. pylori.

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Authors and Affiliations

  1. Center for Animal Resource Development, Wonkwang University, Iksan, 54538, Korea

    Hyun-A Lee, Sunhwa Hong & Okjin Kim

  2. Technlogy Service Division, National Institute of Animal Science, Rural Development Administration, Wanju, Korea

    Ji-Hyun Yoo

  3. Department of Companion Animal and Animal Resources Science, Joongbu University, Geumsan-gun, Korea

    Yungho Chung

  4. Institute of Animal Experiment & Efficacy Evaluation, Wonkwang University, Iksan, Korea

    Okjin Kim

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  1. Hyun-A Lee
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  2. Sunhwa Hong
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Correspondence to Okjin Kim.

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Lee, HA., Hong, S., Yoo, JH. et al. Anti-Helicobacter pylori activity and inhibition of gastritis by Allium hookeri extract. Lab Anim Res 34, 75–79 (2018). https://doi.org/10.5625/lar.2018.34.2.75

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  • DOI: https://doi.org/10.5625/lar.2018.34.2.75

Keywords

Laboratory Animal Research

ISSN: 2233-7660