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Changes of myelin basic protein in the hippocampus of an animal model of type 2 diabetes

Laboratory Animal Research volume 34, pages 176–184 (2018)Cite this article

Abstract

In this study, we observed chronological changes in the immunoreactivity and expression level of myelin basic protein (MBP), one of the most abundant proteins in the central nervous system, in the hippocampus of Zucker diabetic fatty (ZDF) rats and their control littermates (Zucker lean control; ZLC). In the ZLC group, body weight steadily increased with age; the body weight of the ZDF group, however, peaked at 30 weeks of age, and subsequently decreased. Based on the changes of body weight, animals were divided into the following six groups: early (12-week), middle (30-week), and chronic (52-week) diabetic groups and their controls. MBP immunoreactivity was found in the alveus, strata pyramidale, and lacunosum-moleculare of the CA1 region, strata pyramidale and radiatum of the CA3 region, and subgranular zone, polymorphic layer, and molecular layer of the dentate gyrus. MBP immunoreactivity was lowest in the hippocampus of 12-week-old rats in the ZLC group, and highest in 12-week-old rats in the ZDF group. Diabetes increased MBP levels in the 12-week-old group, while MBP immunoreactivity decreased in the 30-week-old group. In the 52-week-old ZLC and ZDF groups, MBP immunoreactivity was detected in the hippocampus, similar to the 30-week-old ZDF group. Western blot results corroborated with immunohistochemical results. These results suggested that changes in the immunoreactivity and expression of MBP in the hippocampus might be a compensatory response to aging, while the sustained levels of MBP in diabetic animals could be attributed to a loss of compensatory responses in oligodendrocytes.

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Authors and Affiliations

  1. Department of Anatomy and Cell Biology, College of Veterinary Medicine, Seoul National University, Seoul, 08826, Korea

    Sung Min Nam, Woosuk Kim, Jong Whi Kim, Kyu Ri Hahn, Hyo Young Jung, Dae Young Yoo, Je Kyung Seong, In Koo Hwang & Yeo Sung Yoon

  2. Research Institute for Veterinary Science, Seoul National University, Seoul, Korea

    Yeo Sung Yoon

  3. Department of Anatomy, College of Veterinary Medicine, Konkuk University, Seoul, Korea

    Sung Min Nam

  4. Department of Biochemistry and Molecular Biology, Research Institute of Oral Sciences, College of Dentistry, Gangneung-Wonju National University, Gangneung, Korea

    Hyun Jung Kwon & Dae Won Kim

  5. Department of Anatomy, College of Medicine, Soonchunhyang University, Cheonan, Korea

    Dae Young Yoo

  6. KMPC (Korea Mouse Phenotyping Center), Seoul National University, Seoul, Korea

    Je Kyung Seong & Yeo Sung Yoon

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Nam, S.M., Kwon, H.J., Kim, W. et al. Changes of myelin basic protein in the hippocampus of an animal model of type 2 diabetes. Lab Anim Res 34, 176–184 (2018). https://doi.org/10.5625/lar.2018.34.4.176

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Laboratory Animal Research

ISSN: 2233-7660