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Hyperglycemia aggravates decrease in alpha-synuclein expression in a middle cerebral artery occlusion model

Laboratory Animal Research volume 34, pages 195–202 (2018)Cite this article

Abstract

Hyperglycemia is one of the major risk factors for stroke. Hyperglycemia can lead to a more extensive infarct volume, aggravate neuronal damage after cerebral ischemia. α-Synuclein is especially abundant in neuronal tissue, where it underlies the etiopathology of several neurodegenerative diseases. This study investigated whether hyperglycemic conditions regulate the expression of α-synuclein in middle cerebral artery occlusion (MCAO)-induced cerebral ischemic injury. Male Sprague-Dawley rats were treated with streptozotocin (40 mg/kg) via intraperitoneal injection to induce hyperglycemic conditions. MCAO were performed four weeks after streptozotocin injection to induce focal cerebral ischemia, and cerebral cortex tissues were obtained 24 hours after MCAO. We confirmed that MCAO induced neurological functional deficits and cerebral infarction, and these changes were more extensive in diabetic animals compared to non-diabetic animals. Moreover, we identified a decrease in α-synuclein after MCAO injury. Diabetic animals showed a more serious decrease in α-synuclein than non-diabetic animals. Western blot and reverse-transcription PCR analyses confirmed more extensive decreases in α-synuclein expression in MCAO-injured animals with diabetic condition than these of non-diabetic animals. It is accepted that α- synuclein modulates neuronal cell death and exerts a neuroprotective effect. Thus, the results of this study suggest that hyperglycemic conditions cause more serious brain damage in ischemic brain injuries by decreasing α-synuclein expression.

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  1. Two authors contributed equally to this work.

Authors and Affiliations

  1. Department of Anatomy, College of Veterinary Medicine, Research Institute of Life Science, Gyeongsang National University, 501 Jinjudaero, Jinju, Gyeongnam, 52828, Korea

    Ju-Bin Kang, Dong-Kyun Kim, Dong-Ju Park, Murad-Ali Shah & Phil-Ok Koh

  2. Division of Life Science and Applied Life Science, College of Natural Sciences, Gyeongsang National University, Jinju, Korea

    Myeong-Ok Kim

  3. Department of Endocrine Surgery, Gyeongsang National University School of Medicine and Gyeongsang National University Changwon Hospital, Changwon, Korea

    Eun-Jung Jung & Han-Shin Lee

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  1. Ju-Bin Kang
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Correspondence to Phil-Ok Koh.

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Kang, JB., Kim, DK., Park, DJ. et al. Hyperglycemia aggravates decrease in alpha-synuclein expression in a middle cerebral artery occlusion model. Lab Anim Res 34, 195–202 (2018). https://doi.org/10.5625/lar.2018.34.4.195

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  • DOI: https://doi.org/10.5625/lar.2018.34.4.195

Keywords

  • α-Synuclein
  • hyperglycemia
  • MCAO

Laboratory Animal Research

ISSN: 2233-7660