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[18F]FET PET is a useful tool for treatment evaluation and prognosis prediction of anti-angiogenic drug in an orthotopic glioblastoma mouse model

Laboratory Animal Research volume 34, pages 248–256 (2018)Cite this article

Abstract

O-2-18F-fluoroethyl-l-tyrosine ([18F]FET) has been widely used for glioblastomas (GBM) in clinical practice, although evaluation of its applicability in non-clinical research is still lacking. The objective of this study was to examine the value of [18F]FET for treatment evaluation and prognosis prediction of anti-angiogenic drug in an orthotopic mouse model of GBM. Human U87MG cells were implanted into nude mice and then bevacizumab, a representative anti-angiogenic drug, was administered. We monitored the effect of anti-angiogenic agents using multiple imaging modalities, including bioluminescence imaging (BLI), magnetic resonance imaging (MRI), and positron emission tomography-computed tomography (PET/CT). Among these imaging methods analyzed, only [18F]FET uptake showed a statistically significant decrease in the treatment group compared to the control group (P=0.02 and P=0.03 at 5 and 20 mg/kg, respectively). This indicates that [18F]FET PET is a sensitive method to monitor the response of GBM bearing mice to anti-angiogenic drug. Moreover, [18F]FET uptake was confirmed to be a significant parameter for predicting the prognosis of anti-angiogenic drug (P=0.041 and P=0.007, on Days 7 and 12, respectively, on Pearson’s correlation; P=0.048 and P=0.030, on Days 7 and 12, respectively, on Cox regression analysis). However, results of BLI or MRI were not significantly associated with survival time. In conclusion, this study suggests that [18F]FET PET imaging is a pertinent imaging modality for sensitive monitoring and accurate prediction of treatment response to anti-angiogenic agents in an orthotopic model of GBM.

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Authors and Affiliations

  1. Korea Drug Development Platform using Radio-isotope, Korea Institute of Radiological & Medical Sciences, 75 Nowonro, Nowon-gu, Seoul, 01812, Korea

    Ok-Sun Kim, Jang Woo Park, Eun Sang Lee, Ran Ji Yoo, Jae Hoon Shim & Hye Kyung Chung

  2. College of Veterinary Medicine, Chonbuk National University, Iksan, Korea

    Ok-Sun Kim & Won-Il Kim

  3. Division of Applied RI, Korea Institute of Radiological & Medical Sciences, Seoul, Korea

    Kyo Chul Lee

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Correspondence to Hye Kyung Chung.

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Kim, OS., Park, J.W., Lee, E.S. et al. [18F]FET PET is a useful tool for treatment evaluation and prognosis prediction of anti-angiogenic drug in an orthotopic glioblastoma mouse model. Lab Anim Res 34, 248–256 (2018). https://doi.org/10.5625/lar.2018.34.4.248

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  • DOI: https://doi.org/10.5625/lar.2018.34.4.248

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Laboratory Animal Research

ISSN: 2233-7660