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Comparison of the anesthetic effects of 2,2,2-tribromoethanol on ICR mice derived from three different sources

Laboratory Animal Research volume 34, pages 270–278 (2018)Cite this article

Abstract

This study was conducted to compare the anesthetic effects of 2,2,2-tribromoethanol (TBE, Avertin®) in ICR mice obtained from three different sources. TBE (2.5%) was intraperitoneally injected at three doses: high-dose group (500 mg/kg), intermediate-dose group (250 mg/kg), and low-dose group (125 mg/kg). Anesthesia time, recovery time, end-tidal peak CO2 (ETCO2), mean arterial blood pressure, heart rate, oxygen saturation (SpO2), body temperature, pH, PCO2, and PO2 of the arterial blood were measured. Stable anesthesia was induced by all doses of TBE and the anesthesia time was maintained exhibited dose dependency. No significant differences in anesthetic duration were found among the three different strains. However, the anesthesia time was longer in female than in male mice, and the duration of anesthesia was significantly longer in female than in male mice in the high-dose group. The recovery time was significantly longer for female than male mice in the intermediate- and high-dose groups. In the ICR strains tested, there were no significant differences in the mean arterial blood pressure, SPO2, arterial blood PCO2, and PO2, which decreased after TBE anesthesia, or in heart rate and ETCO2, which increased after TBE anesthesia. In addition, body temperature, blood biochemical markers, and histopathological changes of the liver, kidney, and lung were not significantly changed by TBE anesthesia. These results suggested that ICR mice from different sources exhibited similar overall responses to a single exposure to TBE anesthesia. In conclusion, TBE is a useful drug that can induce similar anesthetic effects in three different strains of ICR mice.

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Author notes

  1. These authors equally contributed to this work

Authors and Affiliations

  1. College of Veterinary Medicine, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu, 41566, Korea

    Mi Ree Lee, Hye Rin Suh, Myeong Whan Kim & Kil Soo Kim

  2. Department of Health and Exercise Science, Korea National Sport University, Seoul, Korea

    Joon Young Cho

  3. Biomedical Science Institute, Changwon National University, Changwon, Korea

    Hyun Keun Song

  4. Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, Korea

    Young Suk Jung

  5. College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, Korea

    Dae Youn Hwang

  6. Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Korea

    Kil Soo Kim

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  1. Mi Ree Lee
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Correspondence to Kil Soo Kim.

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Lee, M.R., Suh, H.R., Kim, M.W. et al. Comparison of the anesthetic effects of 2,2,2-tribromoethanol on ICR mice derived from three different sources. Lab Anim Res 34, 270–278 (2018). https://doi.org/10.5625/lar.2018.34.4.270

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  • DOI: https://doi.org/10.5625/lar.2018.34.4.270

Keywords

  • Anesthesia effect
  • Korl:ICR mice
  • pulse oximeter
  • sex difference
  • tribromoethanol

Laboratory Animal Research

ISSN: 2233-7660