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Nucleotide-binding oligomerization domain 1 is dispensable for host immune responses against pulmonary infection of Acinetobacter baumannii in mice

Laboratory Animal Research volume 34, pages 295–301 (2018)Cite this article

Abstract

Nucleotide-binding domain 1 (Nod1) is a cytosolic receptor that is responsible for the recognition of a bacterial peptidoglycan motif containing meso-diaminophimelic acid. In this study, we sought to identify the role of Nod1 in host defense in vivo against pulmonary infection by multidrug resistant Acinetobacter baumannii. Wildtype (WT) and Nod1-deficient mice were intranasally infected with 3x107 CFU of A. baumannii and sacrificed at 1 and 3 days post-infection (dpi). Bacterial CFUs, cytokines production, histopathology, and mouse β-defensins (mBD) in the lungs of infected mice were evaluated. The production of cytokines in response to A. baumannii was also measured in WT and Nod1-deficient macrophages. The bacterial clearance in the lungs was not affected by Nod1 deficiency. Levels of IL-6, TNF-α, and IL-1β in the lung homogenates were comparable at days 1 and 3 between WT and Nod1-deficient mice, except the TNF-α level at day 3, which was higher in Nod1-deficient mice. There was no significant difference in lung pathology and expression of mBDs (mBD1, 2, 3, and 4) between WT and Nod1-deficient mice infected with A. baumannii. The production of IL-6, TNF-α, and NO by macrophages in response to A baumannii was also comparable in WT and Nod1-deficient mice. Our results indicated that Nod1 does not play an important role in host immune responses against A. baumannii infection.

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Authors and Affiliations

  1. Laboratory Animal Medicine, College of Veterinary Medicine, Chonnam National University, 77 Yongbongro, Buk-gu, Gwangju, 61186, Korea

    Min-Jung Kang, Jin-A Choi, Joo-Hee Choi, Ah-Ra Jang, Ji-Yeon Park, Jae-Hun Ahn, Tae-Sung Lee, Dong-Yeon Kim & Jong-Hwan Park

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  1. Min-Jung Kang
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Correspondence to Jong-Hwan Park.

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Kang, MJ., Choi, JA., Choi, JH. et al. Nucleotide-binding oligomerization domain 1 is dispensable for host immune responses against pulmonary infection of Acinetobacter baumannii in mice. Lab Anim Res 34, 295–301 (2018). https://doi.org/10.5625/lar.2018.34.4.295

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  • DOI: https://doi.org/10.5625/lar.2018.34.4.295

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Laboratory Animal Research

ISSN: 2233-7660