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Genetic and morphometric characteristics of Korean wild mice (KWM/Hym) captured at Chuncheon, South Korea


Laboratory inbred mice are used widely and commonly in biomedical research, but inbred mice do not have a big enough gene pool for the research. In this study, genetic and morphometric analyses were performed to obtain data on the characteristics of a newly developing inbred strain (KWM/Hym) captured from Chuncheon, Korea. All of five Korean wild male mice have the zinc-finger Y (ZfY) gene. Also, all of 19 Korean wild mice used in this analysis have the AKV-type murine leukemia virus gene, indicating that Korean wild mice might be Mus musculus musculus. To identify the genetic polymorphism in KWM/Hym, SNP analysis was performed. In a comparison with 28 SNP markers, there was a considerable difference between KWM/Hym and several inbred strains. The homogeneity between KWM/Hym and the inbred strains was as follows: C57BL/6J (39.3%), BALB/c AJic (42.9%), and DBA/2J (50%). KWM/Hym is most similar to the PWK/PhJ inbred strain (96.4%) derived from wild mice (Czech Republic). To identify the morphometric characteristics of KWM/Hym, the external morphology was measured. The tail ratio of male and female was 79.60±3.09 and 73.55±6.14%, respectively. KWM/Hym has short and agouti-colored hairs and its belly is white with golden hair. Taking these results together, KWM/Hym, a newly developing inbred mouse originated from wild mouse, might be use as new genetic resources to overcome the limitations of the current laboratory mice.


  1. 1.

    Bedell MA, Jenkins NA, Copeland NG. Mouse models of human disease. Part I: techniques and resources for genetic analysis in mice. Genes Dev 1997; 11(1): 1–10.

    CAS  Article  Google Scholar 

  2. 2.

    The mouse in biomedical research, 2nd Edition. Academic Press, 2006.

  3. 3.

    Abe K, Noguchi H, Tagawa K, Yuzuriha M, Toyoda A, Kojima T, Ezawa K, Saitou N, Hattori M, Sakaki Y, Moriwaki K, Shiroishi T. Contribution of Asian mouse subspecies Mus musculus molossinus to genomic constitution of strain C57BL/6J, as defined by BAC-end sequence-SNP analysis. Genome Res 2004; 14(12): 2439–2447.

    Article  Google Scholar 

  4. 4.

    Reardon S. Frustrated Alzheimer’s researchers seek better lab mice. Nature 2018; 563(7733): 611–612.

    CAS  Article  Google Scholar 

  5. 5.

    Schwarz E, Schwarz HK. The wild and commensal stocks of the house mouse, Mus musculus. J Mammal 1943; 24: 59–72.

    Article  Google Scholar 

  6. 6.

    Boursot P, Auffray JC, Britton-Davidian J, Bonhomme F. The evolution of house mice. Annu Rev Ecol Syst 1993; 24: 119–152.

    Article  Google Scholar 

  7. 7.

    Bonhomme F, Catalan J, Britton-Davidian J, Chapman VM, Moriwaki K, Nevo E, Thaler L. Biochemical diversity and evolution in the genus Mus. Biochem Genet 1984; 22(3–4): 275–303.

    CAS  Article  Google Scholar 

  8. 8.

    Yonekawa H, Moriwaki K, Gotoh O, Hayashi JI, Watanabe J, Miyashita N, Petras ML, Tagashira Y. Evolutionary relationships among five subspecies of Mus musculus based on restriction enzyme cleavage patterns of mitochondrial DNA. Genetics 1981; 98(4): 801–816.

    CAS  PubMed  PubMed Central  Google Scholar 

  9. 9.

    Suzuki H, Miyashita N, Moriwaki K, Kominami R, Muramatsu M, Kanehisa T, Bonhomme F, Petras ML, Yu ZC, Lu DY. Evolutionary implication of heterogeneity of the nontranscribed spacer region of ribosomal DNA repeating units in various subspecies of Mus musculus. Mol Biol Evol 1986; 3(2): 126–137.

    CAS  PubMed  Google Scholar 

  10. 10.

    Moriwaki K, Shiroishi T, Yonekawa H. Wild mouse from a geneticist’s viewpoint. In: Genetics in Wild Mice. Japan Scientific Societies Press, Tokyo: Karger, 1994; pp 13–25.

    Google Scholar 

  11. 11.

    Lee YH, Park JY, Yun YM, Oh SH, Lee JE, Jin HK, Do SG, Suh JG, Lee CH, Hyun BH, Oh YS, Seong JK. Genetic characteristics of Korean wild mice (Mus musculus spp.) by biochemical marker gene. Lab Anim Res 2000; 16(1): 33–39.

    Google Scholar 

  12. 12.

    Duplantier JM, Orth A, Catalan J, Bonhomme F. Evidence for a mitochondrial lineage originating from the Arabian peninsula in the Madagascar house mouse (Mus musculus). Heredity (Edinb) 2002; 89(2): 154–158.

    CAS  Article  Google Scholar 

  13. 13.

    Petkov PM, Cassell MA, Sargent EE, Donnelly CJ, Robinson P, Crew V, Asquith S, Haar RV, Wiles MV. Development of a SNP genotyping panel for genetic monitoring of the laboratory mouse. Genomics 2004; 83(5): 902–911.

    CAS  Article  Google Scholar 

  14. 14.

    Shao Y, Wang J, Qiao Y, He Y, Cao W. Morphological variability between wild populations and inbred stocks of a Chinese minnow, Gobiocypris rarus. Zoolog Sci 2007; 24(11): 1094–1102.

    Article  Google Scholar 

  15. 15.

    Yom-Tov Y, Geffen E. Geographic variation in body size: the effects of ambient temperature and precipitation. Oecologia 2006; 148(2): 213–218.

    Article  Google Scholar 

  16. 16.

    Berry RJ. Natural history of the house mouse. Field Studies Council, 1970; 3: pp 219–262.

    Google Scholar 

  17. 17.

    Hwang SH, Cho KI, Park JH, Ryoo ZY, Oh YS, Suh JG. Studies on distribution of the Zfy, Fv4r, and AKV-type MuLV genes in Korean wild mice. Lab Anim Res 2005; 21(2): 135–139.

    Google Scholar 

  18. 18.

    Marshall JT. Taxonomy in “The mouse in biomedical research”. Academic Press, 1981; pp 17–26.

    Google Scholar 

  19. 19.

    Auffray C, Tchernov E, Bonhomme F, Giora Heth S, Simson S, Nevo E. Presence and ecological distribution of Mus “spretoides” and Mus musculus domesticus in Israel-Circum-Mediterranean vicariance in the genus Mus. Z. Saugetierkunde 1990; 55: 1–10.

    Google Scholar 

  20. 20.

    Boursot P, Din W, Anand R, Darviche D, Dod B, Von Deimling F, Talwar GP, Bonhomme F. Origin and radiation of the house mouse: mitochondrial DNA phylogeny. J of Evolutionary Biology 1996; 9(4): 391–415.

    CAS  Article  Google Scholar 

  21. 21.

    Barton NH, Keightley PD. Understanding quantitative genetic variation. Nat Rev Genet 2002; 3(1): 11–21.

    CAS  Article  Google Scholar 

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Correspondence to Jun Gyo Suh.

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Nam, H., Kim, Y.Y., Kim, B. et al. Genetic and morphometric characteristics of Korean wild mice (KWM/Hym) captured at Chuncheon, South Korea. Lab Anim Res 34, 311–316 (2018).

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  • Korean wild mouse
  • Mus musculus
  • subspecies
  • polymorphism
  • genetic resources