Fig. 4

Anti-CXCR3 antibody inhibits IL-4C-induced CD8 T cell accumulation in the lungs. After IL-4C injection daily for 7 days, control and IL-4C-treated mice were intraperitoneally injected with 500 µg amounts of anti-CXCR3 Ab, followed by influenza A infection. Lungs were harvested 5 days after infection. A CD44^hi and CD44^low CD8 T cell numbers were determined via flow cytometry. The data are the means ± SDs (n = 4–5/group). B CD44^hi and CD44^low CD4 T cell numbers were determined. The data are the means ± SDs (n = 5/group). (C) Cxcl10 transcript levels were measured using real-time PCR and normalized to the expression levels of the Gapdh gene. The data are means ± SDs (n = 12/group); the x-values are fold changes. n.s, not significant. Statistical analysis was performed using an unpaired t-test. *p < 0.05; **p < 0.01