Fig. 5

IL-4C has no impact on innate cell infiltration or antibody titer. Cells were isolated from the lungs of IL-4 treated and control mice. A The numbers of innate immune cells (alveolar macrophage (AM), inflammatory monocyte (iMono), neutrophil and eosinophil) were counted via flow cytometry on days 1 and 5 after influenza A infection. The data are the means ± SDs (n = 5/group); the x numbers indicate fold changes. B Sera of IL-4-treated and control mice were collected 5 days after influenza A infection, and the anti-PR8 antibody titers were assessed via ELISA. Sera obtained 20 days after influenza infection of naïve mice served as the positive (+) control. C Virus titers in the lungs of each group were analyzed by plaque assay. A pooled graph is shown (n = 12/group). The x-values represent fold change. The data are the means ± SD (n = 9/group). Statistical analysis was performed using an unpaired t-test and Mann–Whitney test. n.s, not significant. *p < 0.0; **p < 0.01