Fig. 3

Biphasic immune responses in the aAD and cAD induction models. Serum samples were obtained 1 day prior to (Day 0) the first DNCB sensitization and again at Day 14 following induction. For the cAD groups, an additional sample was collected at Day 42. The IgG1/IgG2a ratio was calculated by dividing serum level of IgG1 by that of IgG2a, and the IL-4/IFN-γ ratio was calculated by dividing the IL-4 level (pg/ml) by the IFN-γ level (pg/ml), followed by multiplication by 100. Single cell suspensions on spleen cells were prepared on Day 28 for the aAD groups and on Day 42 for the cAD groups, and stimulated with immobilized anti-CD3 mAb for 48 h. Data are presented as the mean ± SEM. * indicates significantly different from the aAD(-)/EtOH group at Day 14 and its respective Day 0 level. ** indicates significantly different from its respective Day 0 level. *** indicates significantly different from the aAD(-)/EtOH at Day 14 level. # indicates significantly different from the aAD(-)/EtOH group. The abbreviations were described as in Figs. 1 and 2