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Comparison of the response using ICR mice derived from three different sources to multiple low-dose streptozotocin-induced diabetes mellitus

Laboratory Animal Research volume 33, pages 150–156 (2017)Cite this article

Abstract

This study was conducted to compare the multiple low-dose streptozotocin (MLDS)-induced diabetic patterns of Korl:ICR, A:ICR, and B:ICR mice obtained from three different sources. Six-week-old male ICR mice were obtained from three difference sources. Korl:ICR mice were kindly provided by the National Institute of Food and Drug Safety Evaluation (NIFDS). The other two groups of ICR mice were purchased from different vendors located in the United States (A:ICR) and Japan (B:ICR). All ICR mice that received MLDS exhibited overt diabetic symptoms throughout the study, and the incidence and development of diabetes mellitus were similar among the three ICR groups. The diabetic mice exhibited hyperglycemia, loss of body weight gain, decreased plasma insulin levels, impaired glucose tolerance, decreased number of insulin-positive cells, and decreased size of β-cells in the pancreas. The diabetes symptoms increased as the blood glucose level increased in the three ICR groups. In particular, the level of blood glucose in the STZ-treated group was higher in Korl:ICR and A:ICR mice than in B:ICR mice. The plasma insulin levels, glucose tolerance, blood chemistry, and morphological appearance of the pancreas were very similar in the ICR groups obtained from the three different sources. In conclusion, our results suggest that Korl:ICR, A:ICR, and B:ICR mice from different sources had similar overall responses to multiple low-dose STZ to induce diabetes mellitus.

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Acknowledgments

This project was supported by a grant from BIOREIN (Laboratory Animal Bio Resources Initiative) from the Ministry of Food and Drug Safety in 2015.

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Authors and Affiliations

  1. College of Veterinary Medicine, Kyungpook National University, 80 Daehakro, Buk-gu, Daegu, 41566, Korea

    Do Yeon Lee, Myeong Hwan Kim, Hye Rin Suh & Kil Soo Kim

  2. Department of Pharmacy, College of Pharmacy, Pusan National University, Busan, 46241, Korea

    Young Suk Jung

  3. Department of Biomaterials Science, College of Natural Resources & Life Science/Life and Industry Convergence Research Institute, Pusan National University, Miryang, 50463, Korea

    Dae Youn Hwang

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  1. Do Yeon Lee
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Correspondence to Kil Soo Kim.

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Lee, D.Y., Kim, M.H., Suh, H.R. et al. Comparison of the response using ICR mice derived from three different sources to multiple low-dose streptozotocin-induced diabetes mellitus. Lab Anim Res 33, 150–156 (2017). https://doi.org/10.5625/lar.2017.33.2.150

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  • DOI: https://doi.org/10.5625/lar.2017.33.2.150

Keywords

  • Korl:ICR mice
  • multiple low-dose streptozotocin
  • diabetes mellitus
  • hyperglycemia

Laboratory Animal Research

ISSN: 2233-7660